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1.

1079
Accesses

Research   Open Access Highly Accessed

Optimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots

Cosette Abdallah, Kjell Sergeant, Christelle Guillier, Eliane Dumas-Gaudot, Céline C Leclercq, Jenny Renaut Proteome Science 2012, 10:37 (6 June 2012)

Abstract | Full text | PDF | PubMed

2.

1028
Accesses

Research   Open Access

Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells

Ariel B Abraham, Robert Bronstein, Emily I Chen, Antonius Koller, Lorenza Ronfani, Mirjana Maletic-Savatic, Stella E Tsirka Proteome Science 2013, 11:18 (27 April 2013)

Abstract | Provisional PDF | PubMed

3.

901
Accesses

Methodology   Open Access

Whole gel processing procedure for GeLC-MS/MS based proteomics

Sander R Piersma, Marc O Warmoes, Meike de Wit, Inge de Reus, Jaco C Knol, Connie R Jiménez Proteome Science 2013, 11:17 (23 April 2013)

Abstract | Full text | PDF | ePUB | PubMed

4.

660
Accesses

Research   Open Access

Proteomics approach combined with biochemical attributes to elucidate compatible and incompatible plant-virus interactions between Vigna mungo and Mungbean Yellow Mosaic India Virus

Subrata Kundu, Dipjyoti Chakraborty, Anirban Kundu, Amita Pal Proteome Science 2013, 11:15 (15 April 2013)

Abstract | Full text | PDF | ePUB | PubMed

5.

629
Accesses

Review   Open Access Highly Accessed

Phosphoprotein analysis: from proteins to proteomes

Frédéric Delom, Eric Chevet Proteome Science 2006, 4:15 (19 July 2006)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

6.

620
Accesses

Retraction   Open Access

Retraction: clinical and technical phosphoproteomic research

Elena López, Isabel López, Antonio Ferreira, Julia Sequí Proteome Science 2013, 11:16 (23 April 2013)

Abstract | Provisional PDF | PubMed

7.

600
Accesses

Methodology   Open Access

Interactions by 2D Gel Electrophoresis Overlap (iGEO): a novel high fidelity approach to identify constituents of protein complexes

Masaaki Yoshigi, Stephen M Pronovost, Julie L Kadrmas Proteome Science 2013, 11:21 (12 May 2013)

Abstract | Provisional PDF | PubMed

8.

577
Accesses

Research   Open Access

Comparative proteome analysis of Tumor necrosis factor α-stimulated human Vascular Smooth Muscle Cells in response to melittin

Hyun-Ji Cho, Jeong-Han Kang, Kwan-Kyu Park, Jung-Yoon Choe, Yoon-Yub Park, Yong-Suk Moon, Il-Kyung Chung, Hyeun-Wook Chang, Cheorl-Ho Kim, Yung Choi, Wun-Jae Kim, Sung-Kwon Moon, Young-Chae Chang Proteome Science 2013, 11:20 (7 May 2013)

Abstract | Full text | PDF | ePUB | PubMed

9.

564
Accesses

Research   Open Access

A SELDI mass spectrometry study of experimental autoimmune encephalomyelitis: sample preparation, reproducibility, and differential protein expression patterns

Sausan Azzam, Laurie Broadwater, Shuo Li, Ernest Freeman, Jennifer McDonough, Roger B Gregory Proteome Science 2013, 11:19 (1 May 2013)

Abstract | Provisional PDF | PubMed

10.

515
Accesses

Research   Open Access

Profiling and annotation of human kidney glomerulus proteome

Zenyui Cui, Yutaka Yoshida, Bo Xu, Ying Zhang, Masaaki Nameta, Sameh Magdeldin, Tomoo Makiguchi, Toshikazu Ikoma, Hidehiko Fujinaka, Eishin Yaoita, Tadashi Yamamoto Proteome Science 2013, 11:13 (8 April 2013)

Abstract | Full text | PDF | ePUB | PubMed

11.

492
Accesses

Methodology   Open Access Highly Accessed

Antibody cross-linking and target elution protocols used for immunoprecipitation significantly modulate signal-to noise ratio in downstream 2D-PAGE analysis

Mirta ML Sousa, Kristian W Steen, Lars Hagen, Geir Slupphaug Proteome Science 2011, 9:45 (4 August 2011)

Abstract | Full text | PDF | PubMed

12.

475
Accesses

Research   Open Access

Pre-analytical stability of the plasma proteomes based on the storage temperature

Sara Pasella, Angela Baralla, Elisabetta Canu, Sara Pinna, James Vaupel, Marta Deiana, Claudio Franceschi, Giovannella Baggio, Angelo Zinellu, Salvatore Sotgia, Giuseppe Castaldo, Ciriaco Carru, Luca Deiana Proteome Science 2013, 11:10 (21 March 2013)

Abstract | Full text | PDF | PubMed

13.

411
Accesses

Methodology   Open Access

An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays

Huifang Guo, Wenbin Liu, Zhenlin Ju, Pheroze Tamboli, Eric Jonasch, Gordon B Mills, Yiling Lu, Bryan T Hennessy, Dimitra Tsavachidou Proteome Science 2012, 10:56 (24 September 2012)

Abstract | Full text | PDF | PubMed

14.

378
Accesses

Research   Open Access Highly Accessed

Sample prep for proteomics of breast cancer: proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers

Lambert CM Ngoka Proteome Science 2008, 6:30 (24 October 2008)

Abstract | Full text | PDF | PubMed

15.

339
Accesses

Research   Open Access Highly Accessed

In-depth analysis of the chicken egg white proteome using an LTQ Orbitrap Velos

Karlheinz Mann, Matthias Mann Proteome Science 2011, 9:7 (7 February 2011)

Abstract | Full text | PDF | PubMed

16.

336
Accesses

Research   Open Access

Plasma proteome changes associated with refractory anemia and refractory anemia with ringed sideroblasts in patients with myelodysplastic syndrome

Pavel Májek, Zuzana Riedelová-Reicheltová, Jiří Suttnar, Klára Pečánková, Jaroslav Čermák, Jan E Dyr Proteome Science 2013, 11:14 (8 April 2013)

Abstract | Full text | PDF | ePUB | PubMed

17.

330
Accesses

Research   Open Access Highly Accessed

MALDI/MS peptide mass fingerprinting for proteome analysis: identification of hydrophobic proteins attached to eucaryote keratinocyte cytoplasmic membrane using different matrices in concert

Florence Gonnet, Gilles Lemaître, Gilles Waksman, Jeanine Tortajada Proteome Science 2003, 1:2 (6 May 2003)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

Use of a combination of matrices in matrix-assisted laser desorption-ionisation mass spectrometry greatly improves the identification of hydrophobic membrane-associated proteins in cell extracts. A different combination suits hydrophilic protein identification.

18.

323
Accesses

Methodology   Open Access Highly Accessed

Optimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach

Guennadi A Khoudoli, Iain M Porter, J Julian Blow, Jason R Swedlow Proteome Science 2004, 2:6 (9 September 2004)

Abstract | Full text | PDF | PubMed

19.

323
Accesses

Research   Open Access

Identification of differentially expressed proteins of Arthrospira (Spirulina) plantensis-YZ under salt-stress conditions by proteomics and qRT-PCR analysis

Huili Wang, Yanmei Yang, Wei Chen, Li Ding, Peizhen Li, Xiaokai Zhao, Xuedong Wang, Aiying Li, Qiyu Bao Proteome Science 2013, 11:6 (30 January 2013)

Abstract | Full text | PDF | PubMed

20.

304
Accesses

Research   Open Access Highly Accessed

Comparative proteomic analysis of early salt stress-responsive proteins in roots of SnRK2 transgenic rice

Myung Hee Nam, Sun Mi Huh, Kyung Mi Kim, Woong June Park, Jong Bok Seo, Kun Cho, Dool Yi Kim, Beom Gi Kim, In Sun Yoon Proteome Science 2012, 10:25 (31 March 2012)

Abstract | Full text | PDF | PubMed

21.

305
Accesses

Research   Open Access

Proteomic analysis of elite soybean Jidou17 and its parents using iTRAQ-based quantitative approaches

Jun Qin, Feng Gu, Duan Liu, Changcheng Yin, Shuangjin Zhao, Hao Chen, Jianan Zhang, Chunyan Yang, Xu Zhan, Mengchen Zhang Proteome Science 2013, 11:12 (26 March 2013)

Abstract | Full text | PDF | PubMed

22.

300
Accesses

Review   Open Access Highly Accessed

Highlights on the capacities of "Gel-based" proteomics

François Chevalier Proteome Science 2010, 8:23 (28 April 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

23.

297
Accesses

Research   Open Access Highly Accessed

Peptides OFFGEL electrophoresis: a suitable pre-analytical step for complex eukaryotic samples fractionation compatible with quantitative iTRAQ labeling

Jérôme Chenau, Sylvie Michelland, Jonathan Sidibe, Michel Seve Proteome Science 2008, 6:9 (26 February 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

24.

296
Accesses

Research   Open Access

Identification of protein functions using a machine-learning approach based on sequence-derived properties

Bum Lee, Moon Shin, Young Oh, Hae Oh, Keun Ryu Proteome Science 2009, 7:27 (9 August 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

25.

292
Accesses

Research   Open Access Highly Accessed

Protein identification from two-dimensional gel electrophoresis analysis of Klebsiella pneumoniae by combined use of mass spectrometry data and raw genome sequences

Wei Wang, Jibin Sun, Manfred Nimtz, Wolf-Dieter Deckwer, An-Ping Zeng Proteome Science 2003, 1:6 (3 December 2003)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

26.

290
Accesses

Research   Open Access

Monitoring post mortem changes in porcine muscle through 2-D DIGE proteome analysis of Longissimus muscle exudate

Alessio Di Luca, Giuliano Elia, Anne Maria Mullen, Ruth M Hamill Proteome Science 2013, 11:9 (20 March 2013)

Abstract | Full text | PDF | ePUB | PubMed

27.

289
Accesses

Research   Open Access Highly Accessed

Proteome analysis of soybean leaves, hypocotyls and roots under salt stress

Hamid Sobhanian, Roya Razavizadeh, Yohei Nanjo, Ali Ehsanpour, Ferdous Jazii, Nasrin Motamed, Setsuko Komatsu Proteome Science 2010, 8:19 (29 March 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

28.

279
Accesses

Research   Open Access Highly Accessed

Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome

Thorsten Stellberger, Roman Häuser, Armin Baiker, Venkata R Pothineni, Jürgen Haas, Peter Uetz Proteome Science 2010, 8:8 (15 February 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

29.

267
Accesses

Research   Open Access

The 3T3-L1 adipocyte glycogen proteome

David Stapleton, Chad Nelson, Krishna Parsawar, Marcelo Flores-Opazo, Donald McClain, Glendon Parker Proteome Science 2013, 11:11 (22 March 2013)

Abstract | Full text | PDF | PubMed

30.

268
Accesses

Methodology   Open Access Highly Accessed

A method for protein extraction from different subcellular fractions of laticifer latex in Hevea brasiliensis compatible with 2-DE and MS

Xuchu Wang, Minjing Shi, Xiuli Lu, Ruifeng Ma, Chenggong Wu, Anping Guo, Ming Peng, Weimin Tian Proteome Science 2010, 8:35 (18 June 2010)

Abstract | Full text | PDF | PubMed

31.

261
Accesses

Research   Open Access

Spatiotemporal expression profiling of proteins in rat sciatic nerve regeneration using reverse phase protein arrays

David J Bryan, C Litchfield, Jeffrey V Manchio, Tanya Logvinenko, Antonia H Holway, John Austin, Ian C Summerhayes, Kimberly M Rieger-Christ Proteome Science 2012, 10:9 (10 February 2012)

Abstract | Full text | PDF | PubMed

32.

256
Accesses

Research   Open Access Highly Accessed

Analysis of intracellular expressed proteins of Mycobacterium tuberculosis clinical isolates

Neelja Singhal, Prashant Sharma, Manish Kumar, Beenu Joshi, Deepa Bisht Proteome Science 2012, 10:14 (1 March 2012)

Abstract | Full text | PDF | PubMed

33.

256
Accesses

Research   Open Access Highly Accessed

Proteomic analysis of total cellular proteins of human neutrophils

Gisele G Tomazella, Idalete da Silva, Helen J Laure, José C Rosa, Roger Chammas, Harald G Wiker, Gustavo A de Souza, Lewis J Greene Proteome Science 2009, 7:32 (31 August 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

34.

248
Accesses

Research   Open Access Highly Accessed

Label-free quantitative proteomics of CD133-positive liver cancer stem cells

Sheng-Ta Tsai, Chih-Chiang Tsou, Wan-Yu Mao, Wei-Chao Chang, Hsin-Ying Han, Wen-Lian Hsu, Chung-Leung Li, Chia-Ning Shen, Chung-Hsuan Chen Proteome Science 2012, 10:69 (21 November 2012)

Abstract | Full text | PDF | PubMed

35.

243
Accesses

Research   Open Access

In-depth proteomic analysis of a mollusc shell: acid-soluble and acid-insoluble matrix of the limpet Lottia gigantea

Karlheinz Mann, Eric Edsinger-Gonzales, Matthias Mann Proteome Science 2012, 10:28 (27 April 2012)

Abstract | Full text | PDF | PubMed

36.

236
Accesses

Review   Open Access Highly Accessed

Clinical proteomics for liver disease: a promising approach for discovery of novel biomarkers

Hirofumi Uto, Shuji Kanmura, Yoichiro Takami, Hirohito Tsubouchi Proteome Science 2010, 8:70 (31 December 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

37.

233
Accesses

Research   Open Access Highly Accessed

Improved proteome coverage by using iTRAQ labelling and peptide OFFGEL fractionation

Emilie Ernoult, Erick Gamelin, Catherine Guette Proteome Science 2008, 6:27 (13 October 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

38.

232
Accesses

Research   Open Access

Comparative proteomic analysis implicates eEF2 as a novel target of PI3Kγ in the MDA-MB-231 metastatic breast cancer cell line

Meizhi Niu, Manuela Klingler-Hoffmann, Julie A Brazzatti, Briony Forbes, Chareeporn Akekawatchai, Peter Hoffmann, Shaun R McColl Proteome Science 2013, 11:4 (15 January 2013)

Abstract | Full text | PDF | PubMed

39.

231
Accesses

Methodology   Open Access Highly Accessed

Molecular weight assessment of proteins in total proteome profiles using 1D-PAGE and LC/MS/MS

Q Rushdy Ahmad, Dat H Nguyen, Mark A Wingerd, George M Church, Martin A Steffen Proteome Science 2005, 3:6 (8 June 2005)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

40.

228
Accesses

Research   Open Access Highly Accessed

Identification of differentially accumulated proteins associated with embryogenic and non-embryogenic calli in saffron (Crocus sativus L.)

Golandam Sharifi, Hassan Ebrahimzadeh, Behzad Ghareyazie, Javad Gharechahi, Elaheh Vatankhah Proteome Science 2012, 10:3 (13 January 2012)

Abstract | Full text | PDF | PubMed

41.

226
Accesses

Methodology   Open Access

A novel method for sample preparation of fresh lung cancer tissue for proteomics analysis by tumor cell enrichment and removal of blood contaminants

Luigi De Petris, Maria Pernemalm, Göran Elmberger, Per Bergman, Lotta Orre, Rolf Lewensohn, Janne Lehtiö Proteome Science 2010, 8:9 (26 February 2010)

Abstract | Full text | PDF | PubMed

42.

222
Accesses

Research   Open Access Highly Accessed

Comprehensive proteomic analysis of human cervical-vaginal fluid using colposcopy samples

Geert Zegels, Geert AA Van Raemdonck, Edmond P Coen, Wiebren AA Tjalma, Xaveer WM Van Ostade Proteome Science 2009, 7:17 (17 April 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

43.

220
Accesses

Research   Open Access Highly Accessed

Proteome analysis of the Escherichia coli heat shock response under steady-state conditions

Svenja Lüders, Claas Fallet, Ezequiel Franco-Lara Proteome Science 2009, 7:36 (21 September 2009)

Abstract | Full text | PDF | PubMed

44.

221
Accesses

Research   Open Access

Wounding, insect chewing and phloem sap feeding differentially alter the leaf proteome of potato, Solanum tuberosum L.

Marc-Olivier Duceppe, Conrad Cloutier, Dominique Michaud Proteome Science 2012, 10:73 (26 December 2012)

Abstract | Full text | PDF | PubMed

45.

218
Accesses

Methodology   Open Access Highly Accessed

Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis

Matthew A Churchward, R Hussain Butt, John C Lang, Kimberly K Hsu, Jens R Coorssen Proteome Science 2005, 3:5 (7 June 2005)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

46.

218
Accesses

Methodology   Open Access Highly Accessed

An automated growth enclosure for metabolic labeling of Arabidopsis thaliana with 13C-carbon dioxide - an in vivo labeling system for proteomics and metabolomics research

Wen-Ping Chen, Xiao-Yuan Yang, Geoffrey L Harms, William M Gray, Adrian D Hegeman, Jerry D Cohen Proteome Science 2011, 9:9 (10 February 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

47.

216
Accesses

Research   Open Access Highly Accessed

Protein profiling of the dimorphic, pathogenic fungus, Penicillium marneffei

Julie M Chandler, Erin R Treece, Heather R Trenary, Jessica L Brenneman, Tressa J Flickner, Jonathan L Frommelt, Zaw M Oo, Megan M Patterson, William T Rundle, Olga V Valle, Thomas D Kim, Gary R Walker, Chester R Cooper Proteome Science 2008, 6:17 (4 June 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

48.

215
Accesses

Research   Open Access

Human giant congenital melanocytic nevus exhibits potential proteomic alterations leading to melanotumorigenesis

Hyoung Kyu Kim, Yong Kyu Kim, In-Sung Song, Sung-Ryul Lee, Seung Hun Jeong, Min Hee Kim, Dae Yun Seo, Nari Kim, Byoung Doo Rhee, Kyoung Soo Ko, Kwan Chul Tark, Chul Gyoo Park, Je-Yoel Cho, Jin Han Proteome Science 2012, 10:50 (20 August 2012)

Abstract | Full text | PDF | PubMed

49.

216
Accesses

Research   Open Access

A data management system for structural genomics

Stéphane Raymond, Nicholas O'Toole, Miroslaw Cygler Proteome Science 2004, 2:4 (21 June 2004)

Abstract | Full text | PDF | PubMed

50.

214
Accesses

Research   Open Access

Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86

William J Hurkman, Charlene K Tanaka, William H Vensel, Roger Thilmony, Susan B Altenbach Proteome Science 2013, 11:8 (22 February 2013)

Abstract | Full text | PDF | PubMed

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