Table 1

Identification of differential phosphoproteins in EGF-stimulated and unstimulated NPC CNE2 cells by 2-D DIGE and MS

No.

Protein name

Gene name

Uniprot accession

Mw

(Da)

pI

Number of

Matched

Sequence

coverage

Score

Ratio

(experiment/control)

Phosphorylation sites

peptides

(%)

Ratio

t test

1

Translation endoplasmic reticulam ATPase (TERA)

VCP

P55072

89950

5.14

16

31%

148

1.75

0.0082

Yes

2

Heat shock cognate 71kDa protein

HSP7C

P11142

71082

5.37

13

38%

118

-1.51

0.018

Yes

3

Keratin,type Ⅱ cytoskeletal 8

KRT8

P05787

53671

5.52

18

43%

139

3.47

0.0035

Yes

4

BAG family molecular chaperone regulator 5

BAG5

Q9UL15

51738

5.76

4

15%

56

-2.51

0.0001

No

5

TUBB protein

TUBB

P07437

50095

4.78

25

62%

278

3.12

0.0009

Yes

6

Vimentin

VIM

P08670

53676

5.06

12

30%

102

1.99

0.0056

Yes

7

Heterogeneous nuclear ribonucleoprotein K

hnRNPK

P61978

51230

5.39

8

31%

86

2.89

0.0005

Yes

8

60KD Heat shock protein

HSP60

P10809

61187

5.70

12

29%

109

2.20

0.008

Yes

9

Keratin,type Ⅱ cytoskeletal 8

KRT8

P05787

53671

5.52

6

36%

85

1.63

0.032

Yes

10

Proliferating cell nuclear antigen

PCNA

P12004

29092

4.57

9

52%

93

2.88

0.0001

Yes

11

F-actin-capping protein subunit beta

CAPZB

P47756

31616

5.36

5

26%

56

2.23

0.009

Yes

12

laminin receptor 1

RSSA

P08865

32947

4.79

7

38%

76

1.76

0.0056

Yes

13

Creatine kinase B-type

CKB

P12277

42902

5.34

7

38%

100

1.99

0.0021

Yes

14

Emerin

EMD

P50402

28994

5.29

6

21%

77

1.71

0.0014

Yes

15

Heterogeneous nuclear ribonucleoproteins C1/C2

hnRNP C1/C2

P07910

33707

4.95

5

18%

68

2.98

0.0008

Yes

16

ATP synthase D chain mitochondrial

ATP5H

O75947

18537

5.21

5

37%

57

2.67

0.005

Yes

17

Prohibitin

PHB

P35232

29843

5.57

7

37%

120

-2.54

0.031

Yes

18

unidentified

1.79

0.002

N/A

19

Elongation factor 1- delta

EEF1D

P29692

32.217

4.9

4

25%

72

1.81

0.026

Yes

20

Anamorsin

CIAPIN1

Q6FI81

34141

5.44

9

39%

139

2.90

0.0001

Yes

21

Annexin A3

ANXA3

P12429

36524

5.63

7

27%

83

2.83

0.0054

Yes

22

Glutathione S-transferase P1

GSTP1

P09211

23569

5.43

6

34%

97

3.11

0.0013

Yes

23

unidentified

1.64

0.026

N/A

24

Keratin,typeⅠcytoskeletal 18

KRT18

P05783

48029

5.34

13

36%

141

-2.86

0.029

Yes

25

Ran-specific GTPase- activating protein

RANBP1

P43487

23310

5.71

6

32%

98

1.89

0.034

Yes

26

unidentified

1.68

0.007

N/A

27

Tropomodulin-3

TMOD3

Q9NYL9

39.595

5.08

8

34%

98

2.61

0.003

Yes

28

Myosin light polypeptide 6

MYL6

P60660

17090

4.56

6

50%

70

2.80

0.0019

Yes

29

Nucleoside diphosphate kinase A (NDKA)

NME1

P15531

17309

5.83

6

48%

105

-1.56

0.0023

Yes

30

Heat shock protein beta-1

HSP27

P04792

22826

5.98

7

47%

84

2.65

0.0001

Yes

31

unidentified

-2.72

0.038

N/A

32

Growth factor receptor-bound protein 2

GRB2

P62993

25,206

5.89

7

39%

75

2.60

0.00028

Yes

33

Glutathione S-transferase P1

GSTP1

P09211

23569

5.43

6

43%

91

3.99

3.2E-06

Yes

34

Peroxiredoxin-2

PRDX2

P32119

22049

5.66

6

54%

98

-1.69

0.042

Yes

35

c-Myc-responsive protein

RCL

O43598

19211

4.97

4

25%

67

2.25

0.009

Yes

36

Protein DJ-1

PARK7

Q99497

19.891

6.33

5

30%

65

2.99

0.0036

Yes

37

3-Hydroxyisobutyrate dehydrogenase mitochondrial

precursor (3HIDH)

HIBADH

P31937

35705

8.38

4

22%

95

-2.55

0.0051

Yes

38

Stathmin

STMN1

P16949

17171

5.77

6

19%

95

2.57

0.0023

Yes

Ruan et al. Proteome Science 2011 9:35   doi:10.1186/1477-5956-9-35