The effect of GSTP1 siRNA transfection on paclitaxel sensitivity in EGF-stimulated CNE2 cells. A, Western blotting analysis showed that transfection of CNE2 cells with GSTP1 siRNA knocked down GSTP1 expression, whereas GSTP1 expression was not significantly suppressed by control siRNA. B, Flow cytometric analysis showed that GSTP1 siRNA transfection could significantly increase cell apoptosis in CNE2 cells incubated with paclitaxel and EGF. C, MTT assay showed that GSTP1 siRNA transfection could significantly decrease the cell viability of CNE2 cells incubated with paclitaxel and EGF. Lipofectamine, cells treated with lipofectmine only.
Ruan et al. Proteome Science 2011 9:35 doi:10.1186/1477-5956-9-35