Validation of EGFR-regulated phosphoproteins by IP-Western blotting. A, IP-Western blotting analysis showing that EGF induces phosphorylation of three identified proteins (ANXA3, KRT8 and KRT18). Try, anti-phosphotyrosine antibody B, IP-Western blotting analysis showing that phospho-EGFR interacts with GSTP1 and GRB2. CBB gel staining serves as loading control (IgG heavy chain).
Ruan et al. Proteome Science 2011 9:35 doi:10.1186/1477-5956-9-35