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Open Access Highly Accessed Research

Proteome analysis of soybean leaves, hypocotyls and roots under salt stress

Hamid Sobhanian123, Roya Razavizadeh14, Yohei Nanjo1, Ali Akbar Ehsanpour4, Ferdous Rastgar Jazii3, Nasrin Motamed2 and Setsuko Komatsu1*

Author Affiliations

1 National Institute of Crop Science, Tsukuba 305-8518, Japan

2 School of Biology, College of Science, University of Tehran, Tehran 14155-6455, Iran

3 National Research Center for Genetic Engineering and Biotechnology, Tehran 14155-6343, Iran

4 Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan 81746-73441, Iran

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Proteome Science 2010, 8:19  doi:10.1186/1477-5956-8-19

Published: 29 March 2010

Abstract

Background

Salinity is one of the most widespread agricultural problems in arid and semi-arid regions that makes fields unproductive, and soil salinization is a serious problem in the entire world. To determine the effects of salt stress on soybean seedlings, a proteomic technique was used.

Results

Soybean plants were exposed to 0, 20, 40, or 80 mM NaCl for one week. The effect of treatment at 20 mM NaCl on plant growth was not severe, at 80 mM NaCl was lethal, and at 40 mM NaCl was significant but not lethal. Based on these results, proteins were extracted from the leaves, hypocotyls and roots of soybean treated with 40 mM NaCl. Nineteen, 22 and 14 proteins out of 340, 330 and 235 proteins in the leaves, hypocotyls and roots, respectively, were up- and down-regulated by NaCl treatment. In leaves, hypocotyls and roots, metabolism related proteins were mainly down-regulated with NaCl treatment. Glyceraldehyde-3-phosphate dehydrogenase was down-regulated in the leaf/hypocotyls, and fructokinase 2 was down-regulated in the hypocotyls/root with NaCl treatment. Stem 31 kDa glycoprotein precursor was up-regulated in all three organs with NaCl treatment. Glyceraldehyde-3-phosphate dehydrogenase was specifically down-regulated at the RNA and protein levels by salt stress.

Conclusion

These results suggest that metabolism related proteins play a role in each organ in the adaptation to saline conditions.