 ResearchIntegral and peripheral association of proteins and protein complexes with Yersinia pestis inner and outer membranesRembert Pieper1 1 J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, Maryland, USA 2 Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, Kentucky, USA
Proteome Science 2009, 7:5doi:10.1186/1477-5956-7-5
Additional filesAdditional file 1: Supplemental Table. Supplemental table with extensive information on Yersinia pestis KIM proteins identified from membrane fractions: protein annotations, functional role categories, differential 2D gel display and mass spectrometry data. Format: XLS Size: 380KB Download file This file can be viewed with: Microsoft Excel Viewer Additional file 2: Table 1. Table lists all identified Yersinia pestis KIM6+ membrane-associated proteins with 2D gel spot numbers, locus tags, gene names, their detection in high Mr membrane fractions, their categorization, functional role descriptions, and enrichment factors in membrane fractions (EM and EIM values). Format: DOC Size: 1.4MB Download file This file can be viewed with: Microsoft Word Viewer Additional file 3: Supplemental Information (Figure Two). Protein display of the high salt-extracted membrane fraction isolated from Y. pestis KIM6+ cells grown at 26°C (pH range of 4–6.5). Format: TIFF Size: 2.5MB Download file Additional file 4: Supplemental Information (Figure Three). Protein display of the high salt-extracted membrane fraction isolated from Y. pestis KIM6+ cells grown at 37°C (pH range of 4–9). Format: TIFF Size: 3.1MB Download file Additional file 5: Supplemental Information (Figure Four). Protein display of the high pH-extracted membrane fraction isolated from Y. pestis KIM6+ cells grown at 26°C (pH range of 4–6.5). Format: TIFF Size: 3.1MB Download file Additional file 6: Supplemental Information (Figure Five). Protein display of urea/sulfobetaine-14-extracted membrane fraction isolated from Y. pestis KIM6+ cells grown in low phosphate media at 26°C (pH range of 4–6.5). Format: TIFF Size: 2.8MB Download file Additional file 7: Supplemental Information (Figure Six). Protein display of urea/sulfobetaine-14-extracted membrane fraction isolated from Y. pestis KIM6+ cells grown in low phosphate media at 37°C (pH range of 4–6.5). Format: TIFF Size: 2.4MB Download file Additional file 8: Supplemental Information (Figure Seven). Protein display of urea/amidosulfobetaine-14-extracted membrane fraction isolated from Y. pestis KIM6+ cells grown to stationary phase at 26°C (pH range of 4–7). Format: TIFF Size: 3.4MB Download file Additional file 9: Supplemental Information (Figure Eight). Protein display of urea/amidosulfobetaine-14-extracted membrane fraction isolated from Y. pestis KIM6+ cells grown at 37°C (pH range of 6–10). Format: TIFF Size: 2.2MB Download file Additional file 10: Supplemental Information (Figure One). Protein display of the high salt-extracted cell surface fraction isolated from Y. pestis KIM6+ cells grown at 37°C (pH range 3.5–10). Format: TIFF Size: 2.6MB Download file |
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