Proteins recruited by SH3 domains of Ruk/CIN85 adaptor identified by LC-MS/MS

doi:10.1186/1477-5956-7-21

Proteome Science

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Havrylov S
Rzhepetskyy Y
Malinowska A
Drobot L
Redowicz MJ

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Redowicz MJ
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Proteins recruited by SH3 domains of Ruk/CIN85 adaptor identified by LC-MS/MS

Serhiy Havrylov¹ , Yuriy Rzhepetskyy¹ , Agata Malinowska² , Lyudmyla Drobot¹^,3 and Maria Jolanta Redowicz¹

¹Laboratory of Molecular Basis of Cell Motility, Nencki Institute of Experimental Biology, 3 Pasteur str., 02-093 Warsaw, Poland

²Laboratory of Mass Spectrometry, Institute of Biochemistry and Biophysics, 5a Pawinskiego str., 02-106 Warsaw, Poland

³Palladin Institute of Biochemistry, 9 Leontovich str., Kyiv 01601, Ukraine

author email corresponding author email

Proteome Science 2009, 7:21doi:10.1186/1477-5956-7-21


Published:	16 June 2009

Additional files

Additional file 1:

Modular organisation of Ruk/CIN85 and representative SDS-PAGE gel resulting from GST pull-down experiment. A. Modular organisation of Ruk/CIN85 adaptor molecule. B. Representative SDS-PAGE gel loaded with 1/5 of each sample resulting from GST pull-down experiment. For the LC-MS/MS analysis proteins were separated on a parallel SDS-PAGE gel loaded with 4/5 of each sample. Blank tracks were left between the loaded lanes to avoid cross-contamination. Locations of the excised gel pieces are outlined with colour boxes. Three corresponding gel slices with the size ranges of 75 – 175 kDa, 45 – 75 kDa and 33 – 45 kDa were excised from each lane.

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Additional file 2:

Proteins recruited by the SH3 domains of Ruk/CIN85 identified by the LC-MS/MS. The table lists the proteins recruited by the SH3 domains of Ruk/CIN85 identified by the LC-MS/MS. GI – protein genbank identifier; Name – short protein name; Sample – sample where protein was identified (refer to Additional file 1 for details); Score – Mascot protein score; Mr – protein molecular weight; u.p. – number of unique peptides matched to protein sequence; % c. – % of protein sequence covered by matched peptides; Uniprot – protein accession number in UniProt Knowledgebase. The long and/or alternative names, as well as all additional genbank identifiers for the identified proteins are listed in the two last columns.

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Additional file 3:

Long names of module/domain abbreviations mentioned in the Table 1. The table lists the long names of modules/domain abbreviations mentioned in the Table 1.

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Additional file 4:

Circular phylogram of all the SH3 domains found in mice and men. The figure shows the circular phylogram of all the SH3 domains found in mice and men. The clade harbouring the SH3 domains of Ruk/CIN85 is indicated in green. Magnify.

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Additional file 5:

Scansite matrices used for prediction of potential recognition consensus sites for the SH3 domains of Ruk/CIN85 in the identified proteins. The data provided represent the Scansite matrices used for prediction of potential recognition consensus sites for the SH3 domains of Ruk/CIN85 in the identified proteins.

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Additional file 6:

ENSEMBL IDs of the genes encoding the identified proteins. The table lists ENSEMBL IDs of the genes encoding the identified proteins.

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This file can be viewed with: Microsoft Excel Viewer