Table 3 |
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|
Overview of studies performed on human CVF. |
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|
Study |
Samples |
Part of sample analyzed |
Separation method |
MS method |
|
|
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|
Venkataraman et al., 2005[29] |
Undiluted CVF collected in cup from healthy women (postmenarcheal, pre-menopausal) |
Cationic fraction |
2D-PAGE (1D: AU-PAGE; 2D: Tricine- SDS-PAGE) |
MALDI-TOF-TOF |
|
|
||||
|
Di Quinzio et al., 2007[26] |
Swabs from pregnant women (37 weeks gestation) |
Only protein spots identified common to five gels |
2D-PAGE (1stD: IEF; 2ndD SDS-PAGE) followed by RP-LC |
MALDI-TOF or ESI-linear IT |
|
|
||||
|
Dasari et al., 2007[24] |
Swabs from pregnant women (18,5 weeks gestation as mean) |
Whole CVF |
1D-SDS-PAGE followed by offline 2D(SCX/RP)-LC |
ESI-Q-TOF |
|
|
||||
|
Tang et al., 2007[11] |
Washings from clinically normal women; 7 washings from women infected with Candida spp. |
Whole CVF |
2D-PAGE (1stD: IEF; 2ndD SDS-PAGE) |
MALDI-TOF-TOF |
|
|
||||
|
Shaw et al., 2007[28] |
Gauze from healthy women |
Whole CVF |
1D-SDS-PAGE or SCX-LC both followed by RP-LC |
ESI-linear IT |
|
|
||||
|
Pereira et al., 2007[27] |
Swabs from pregnant women (15.8–35.9 weeks gestation) |
Whole CVF |
2D-DIGE or MudPIT(SCX/RP)-LC |
ESI-Q-TOF |
|
|
||||
|
Klein et al., 2008[25] |
Swabs from pregnant women (30.5 weeks gestation as mean) |
Whole CVF |
RP-LC |
ESI-IT |
|
|
||||
|
This study |
Washings from HPV-infected women |
Whole CVF |
Ultrafiltration or C4-LC protein fractionation/C18-LC peptide separation |
MALDI-TOF-TOF |
|
|
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|
For each study the following information is presented: 1) the nature of the samples which were used in the study (sample collection method and patient physiology), 2) whether the study focused on a particular part of the sample or analyzed the whole CVF, 3) the methods used to separate proteins and peptides and 4) MS method used for analysis. |
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|
Zegels et al. Proteome Science 2009 7:17 doi:10.1186/1477-5956-7-17 |
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