Table 3

Overview of studies performed on human CVF.

Study

Samples

Part of sample analyzed

Separation method

MS method


Venkataraman et al., 2005[29]

Undiluted CVF collected in cup from healthy women (postmenarcheal, pre-menopausal)

Cationic fraction

2D-PAGE (1D: AU-PAGE; 2D: Tricine- SDS-PAGE)

MALDI-TOF-TOF


Di Quinzio et al., 2007[26]

Swabs from pregnant women (37 weeks gestation)

Only protein spots identified common to five gels

2D-PAGE (1stD: IEF; 2ndD SDS-PAGE) followed by RP-LC

MALDI-TOF or ESI-linear IT


Dasari et al., 2007[24]

Swabs from pregnant women (18,5 weeks gestation as mean)

Whole CVF

1D-SDS-PAGE followed by offline 2D(SCX/RP)-LC

ESI-Q-TOF


Tang et al., 2007[11]

Washings from clinically normal women; 7 washings from women infected with Candida spp.

Whole CVF

2D-PAGE (1stD: IEF; 2ndD SDS-PAGE)

MALDI-TOF-TOF


Shaw et al., 2007[28]

Gauze from healthy women

Whole CVF

1D-SDS-PAGE or SCX-LC both followed by RP-LC

ESI-linear IT


Pereira et al., 2007[27]

Swabs from pregnant women (15.8–35.9 weeks gestation)

Whole CVF

2D-DIGE or MudPIT(SCX/RP)-LC

ESI-Q-TOF


Klein et al., 2008[25]

Swabs from pregnant women (30.5 weeks gestation as mean)

Whole CVF

RP-LC

ESI-IT


This study

Washings from HPV-infected women

Whole CVF

Ultrafiltration or C4-LC protein fractionation/C18-LC peptide separation

MALDI-TOF-TOF


For each study the following information is presented: 1) the nature of the samples which were used in the study (sample collection method and patient physiology), 2) whether the study focused on a particular part of the sample or analyzed the whole CVF, 3) the methods used to separate proteins and peptides and 4) MS method used for analysis.

Zegels et al. Proteome Science 2009 7:17   doi:10.1186/1477-5956-7-17

Open Data