|
Spot intensities of proteins present on the array.a |
|||||||||
| Cow 5902 |
Cow 5904 |
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|
|
|||||||||
| Spot Locb |
Protein |
Day 0 |
Day 70 |
Day 194 |
Day 321 |
Day 0 |
Day 70 |
Day 194 |
Day 321 |
|
|
|||||||||
| A7 |
MAP0075 |
0 |
4.472 |
21.431 |
21.122 |
0 |
0 |
27.612 |
21.36 |
| A12 |
MAP0087-his |
0.978 |
0 |
0 |
0 |
1.112 |
1.882 |
0 |
1.228 |
| A5 |
MAP0105c |
0 |
6.929 |
3.867 |
5.096 |
1.186 |
2.321 |
10.106 |
1.813 |
| B5 |
MAP0182c |
0 |
5.407 |
0 |
1.924 |
1.177 |
2.409 |
1.617 |
22.366 |
| C4 |
MAP0216 |
0.652 |
3.779 |
1.296 |
1.395 |
2.175 |
3.444 |
0 |
0 |
| G4 |
MAP0261c |
2.395 |
2.287 |
3.487 |
5.668 |
1.097 |
3.146 |
2.397 |
2.496 |
| E8 |
MAP0389 |
0 |
7.809 |
3.163 |
1.817 |
0 |
1.533 |
16.751 |
5.529 |
| F8 |
MAP0736 |
0.734 |
0 |
0 |
0 |
0 |
0 |
1.446 |
0 |
| A1 |
MAP0852 |
2.257 |
3.747 |
2.908 |
1.147 |
2.564 |
4.661 |
2.709 |
1.537 |
| B1 |
MAP0853 |
2.144 |
3.734 |
5.095 |
8.497 |
1.961 |
4.158 |
3.239 |
2.649 |
| C1 |
MAP0855 |
2.538 |
3.387 |
4.552 |
7.898 |
1.716 |
2.248 |
2.132 |
0 |
| D1 |
MAP0857c |
2.709 |
6.445 |
21.867 |
37.718 |
1.045 |
1.602 |
23.425 |
15.825 |
| E1 |
MAP0858 |
3.005 |
3.882 |
1.775 |
3.999 |
1.978 |
0 |
0 |
0 |
| F1 |
MAP0859c |
2.821 |
4.137 |
2.87 |
3.745 |
1.909 |
0 |
2.803 |
0 |
| G1 |
MAP0860c |
2.996 |
0 |
5.531 |
12.743 |
1.592 |
0 |
7.669 |
6.676 |
| H1 |
MAP0861 |
5.074c |
0 |
3.407 |
3.053 |
1.546 |
4.05 |
3.111 |
3.52 |
| A2 |
MAP0862 |
1.795 |
40.036 |
16.196 |
7.997 |
2.796 |
11.451 |
30.918 |
12.422 |
| B2 |
MAP0863 |
1.518 |
5.524 |
3.174 |
4.592 |
2.057 |
4.891 |
3.274 |
1.473 |
| C2 |
MAP0864 |
1.255 |
3.44 |
1.781 |
1.726 |
2.016 |
3.56 |
0 |
0 |
| D2 |
MAP0865 |
1.909 |
18.708 |
32.138 |
44.846 |
1.317 |
4.386 |
31.361 |
35.251 |
| E2 |
MAP0866 |
2.346 |
7.051 |
2.67 |
7.116 |
1.315 |
3.561 |
4.052 |
6.009 |
| G8 |
MAP0904 |
1.678 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
| G5 |
MAP0961c |
1.797 |
14.366 |
26.5 |
37.546 |
0.78 |
2.103 |
33.046 |
42.877 |
| A11 |
MAP1087 |
0.753 |
53.179 |
51.634 |
70.968 |
0.874 |
33.469 |
75.392 |
68.964 |
| B11 |
MAP1121c |
0.758 |
2.438 |
0 |
2.094 |
0.699 |
3.364 |
0 |
1.848 |
| A10 |
MAP1174c |
0 |
2.301 |
3.155 |
4.411 |
0.623 |
1.921 |
5.81 |
4.955 |
| C11 |
MAP1204 |
0.78 |
40.8 |
43.856 |
55.046 |
0 |
28.367 |
61.122 |
58.985 |
| F2 |
MAP1233 |
2.568 |
12.375 |
22.507 |
32.493 |
1.932 |
3.212 |
24.157 |
30.447 |
| C5 |
MAP1345 |
0 |
3.339 |
1.861 |
1.018 |
1.147 |
0 |
3.413 |
2.389 |
| D5 |
MAP1388 |
0 |
2.281 |
0 |
0 |
0.429 |
2.835 |
0 |
0 |
| E5 |
MAP1416c |
0 |
1.647 |
0 |
0 |
0.791 |
3.791 |
0 |
0 |
| F5 |
MAP1417c |
1.052 |
14.882 |
4.095 |
6.212 |
0.955 |
3.979 |
7.614 |
3.521 |
| D4 |
MAP1609c |
0.733 |
3.916 |
0 |
0 |
1.003 |
5.292 |
0 |
0 |
| G2 |
MAP1636c |
3.286 |
6.944 |
13.249 |
17.272 |
1.701 |
0 |
17.226 |
12.06 |
| B4 |
MAP1643 |
0 |
4.155 |
0 |
1.534 |
1.827 |
0 |
0 |
0 |
| H8 |
MAP1655c |
2.788 |
0 |
0 |
0 |
0 |
2.049 |
0 |
0 |
| G6 |
MAP1730c |
1.147 |
29.831 |
43.872 |
52.35 |
0 |
12.272 |
57.799 |
57.47 |
| B10 |
MAP1931c |
0 |
2.917 |
0 |
2.217 |
0.511 |
2.886 |
2.077 |
3.339 |
| H5 |
MAP2077c |
3.833 |
0 |
2.784 |
9.443 |
1.385 |
0 |
3.63 |
9.221 |
| D11 |
MAP2116c |
1.418 |
29.88 |
33.215 |
42.633 |
0.568 |
11.24 |
51.336 |
47.918 |
| F4 |
MAP2121c |
1.76 |
4.158 |
1.373 |
1.513 |
0.969 |
3.005 |
0 |
0 |
| B12 |
MAP2121c-his |
1.247 |
0 |
0 |
0 |
0.889 |
2.172 |
21.226 |
2.155 |
| H2 |
MAP2151 |
4.136 |
1.919 |
12.688 |
21.897 |
1.571 |
3.402 |
24.619 |
20.889 |
| F12 |
MAP2155 |
2.434 |
0 |
1.385 |
0 |
1.251 |
2.039 |
0 |
2.105 |
| G12 |
MAP2156 |
3.731 |
2.327 |
0 |
2.12 |
1.357 |
2.647 |
5.42 |
5.113 |
| H6 |
MAP2157 |
3.504 |
0 |
0 |
0 |
0.751 |
1.532 |
1.51 |
2.683 |
| A3 |
MAP2158 |
1.403 |
3.487 |
4.2 |
3.396 |
3.187 |
1.704 |
6.682 |
2.204 |
| A9 |
MAP2182c |
0 |
19.101 |
7.349 |
8.933 |
0 |
0 |
27.935 |
12.096 |
| E11 |
MAP2231 |
1.846 |
3.732 |
11.54 |
15.671 |
1.166 |
0 |
17.203 |
18.933 |
| B9 |
MAP2360c |
0 |
3.168 |
0 |
0 |
0 |
2.766 |
0 |
0 |
| C10 |
MAP2380 |
0.772 |
2.139 |
1.198 |
0 |
0 |
0 |
1.968 |
3.225 |
| D10 |
MAP2657 |
0 |
22.372 |
8.29 |
3.68 |
0 |
15.937 |
39.178 |
25.032 |
| B7 |
MAP2663c |
0 |
9.594 |
3.661 |
2.248 |
0.868 |
0 |
4.9 |
2.717 |
| A6 |
MAP2676c |
0 |
3.291 |
1.406 |
0 |
0 |
0 |
2.036 |
0 |
| D7 |
MAP2734 |
2.726 |
9.109 |
11.031 |
9.269 |
0 |
0 |
27.935 |
18.79 |
| C7 |
MAP2740 |
0 |
1.736 |
6.447 |
18.061 |
0.507 |
0 |
6.295 |
10.804 |
| B6 |
MAP2751 |
0 |
3.157 |
1.101 |
2.373 |
0.8 |
0 |
1.729 |
2.206 |
| C6 |
MAP2753 |
0 |
1.909 |
0 |
2.176 |
0.624 |
0 |
1.701 |
2.585 |
| D6 |
MAP2761c |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
| E6 |
MAP2762c |
0 |
0 |
0 |
0 |
0 |
2.397 |
0 |
0 |
| B3 |
MAP2764c |
1.472 |
2.739 |
1.969 |
5.874 |
2.13 |
5.442 |
2.039 |
1.955 |
| H11 |
MAP2765c |
4.816 |
0 |
0 |
0 |
0 |
4.799 |
0 |
0 |
| C3 |
MAP2767c |
1.257 |
3.507 |
2.303 |
7.019 |
1.982 |
4.347 |
1.92 |
2.722 |
| F6 |
MAP2963c |
0 |
0 |
0 |
0 |
0 |
2.204 |
0 |
0 |
| C12 |
MAP3084c-his |
1.313 |
0 |
0 |
0 |
0.999 |
2.026 |
0 |
1.982 |
| E10 |
MAP3121 |
1.079 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
| C9 |
MAP3129 |
1.406 |
2.366 |
0 |
1.206 |
0 |
0 |
1.731 |
2.865 |
| E7 |
MAP3131 |
0 |
0 |
0 |
0 |
0 |
0 |
1.404 |
1.713 |
| G11 |
MAP3155c |
2.783 |
2.553 |
2.725 |
3.841 |
1.435 |
2.237 |
2.226 |
4.865 |
| F7 |
MAP3434 |
0.832 |
1.401 |
8.312 |
19.522 |
0.508 |
0 |
33.289 |
14.418 |
| D3 |
MAP3437c |
1.674 |
3.321 |
1.29 |
1.587 |
1.359 |
1.965 |
0 |
0 |
| E4 |
MAP3531c |
0.926 |
7.373 |
2.472 |
4.313 |
0.558 |
2.834 |
1.374 |
0 |
| G7 |
MAP3734c |
1.085 |
2.238 |
2.719 |
17.809 |
0 |
0 |
10.914 |
20.81 |
| E3 |
MAP3735c |
1.944 |
4.897 |
5.078 |
18.618 |
1.326 |
3.368 |
3.887 |
4.502 |
| F3 |
MAP3743 |
2.113 |
23.874 |
16.282 |
16.773 |
1.28 |
6.953 |
21.309 |
22.067 |
| H7 |
MAP3751 |
2.991 |
0 |
0 |
0 |
0 |
0 |
2.499 |
3.033 |
| D9 |
MAP3753 |
0 |
2.912 |
7.043 |
3.212 |
0 |
0 |
18.95 |
15.247 |
| A8 |
MAP3761c |
0 |
2.473 |
14.931 |
23.178 |
0 |
0 |
18.02 |
21.264 |
| G3 |
MAP3771 |
2.81 |
2.229 |
1.344 |
2.528 |
0.953 |
1.997 |
0 |
0 |
| H3 |
MAP3817c |
3.836 |
2.526 |
10.298 |
22.826 |
1.101 |
1.552 |
15.736 |
19.901 |
| E9 |
MAP3833c |
1.078 |
0 |
2.07 |
2.755 |
0 |
0 |
3.676 |
4.636 |
| A4 |
MAP3840 |
0.823 |
4.968 |
3.842 |
4.521 |
1.403 |
0 |
10.083 |
0 |
| F9 |
MAP3902c |
1.095 |
0 |
0 |
0 |
0 |
2.376 |
0 |
0 |
| G9 |
MAP3903c |
1.755 |
1.355 |
0 |
1.027 |
0 |
1.772 |
0 |
0 |
| H9 |
MAP3954 |
3.426 |
0 |
0 |
1.969 |
0 |
7.584 |
1.612 |
3.629 |
| F11 |
MAP4014 |
2.266 |
2.035 |
5.415 |
8.98 |
0.545 |
0 |
5.293 |
8.043 |
| F10 |
MAP4025 |
1.474 |
25.139 |
34.876 |
39.022 |
0 |
13.413 |
49.655 |
47.012 |
| B8 |
MAP4129 |
0 |
1.489 |
0 |
1.363 |
0 |
0 |
1.435 |
2.585 |
| C8 |
MAP4198 |
0 |
1.536 |
1.517 |
6.817 |
0 |
0 |
5.431 |
6.175 |
| G10 |
MAP4199 |
1.919 |
1.688 |
0 |
0 |
0.821 |
2.339 |
0 |
0 |
| D8 |
MAP4207c |
1.981 |
1.691 |
1.872 |
1.364 |
0 |
0 |
3.699 |
5.632 |
| H10 |
MAP4228 |
4.373 |
0 |
0 |
0 |
0 |
3.122 |
0 |
0 |
| H4 |
MBP-LacZ |
4.128 |
0 |
0 |
1.722 |
1.303 |
0 |
0 |
0 |
| D12 |
PBS bufferd |
1.676 |
0 |
1.236 |
0 |
0.857 |
2.322 |
0 |
2.003 |
| E12 |
Sonicatee |
2.673 |
0 |
0 |
0 |
1.287 |
1.936 |
2.963 |
3.523 |
| H12 |
Sonicate |
5.01 |
1.359 |
0 |
0 |
1.366 |
4.337 |
3.002 |
4.472 |
|
aListed are the intensity values for each spot arranged by ascending MAP number. Measurements were made using a consistent 1804 pixel area circle to record spot density using the Adobe Photoshop CS3 Extended application. bLoc is location on the dot blot array. cThe strongest intensity for each time point is shown in bold. dPBS buffer in spot D12 represents the spotting buffer only control. eSonicate is a sonicated whole cell lysate of M. avium subsp paratuberculosis. | |||||||||
Bannantine et al. Proteome Science 2008 6:5 doi:10.1186/1477-5956-6-5 |
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