Table 1

Mycobacterium avium subsp paratuberculosis proteins used in this study
Protein namea
 Concentration (mg/ml)b
 Gene sizec
 Amino acidsd
 Predicted function
MAP sonicate
 1.0
 NAe
 NA
 NA
MAP0075
 0.18
 423
 140
 conserved small membrane protein
MAP0087-his
 1.0
 717
 238
 probable secreted protein
MAP0105c
 1.24
 1058
 352/889
 hypothetical protein
MAP0182c
 0.67
 411
 136
 conserved hypothetical protein
MAP0216
 1.66
 1028
 342/347
 antigen 85A, mycolyltransferase
MAP0261c
 0.49
 465
 154/161
 19 kDa protein
MAP0389
 2.04
 947
 315/337
 diarylpropane peroxidase (EC 1.11.1.14) [Nostoc]
MAP0736
 5.73
 744
 247
 hypothetical protein
MAP0852
 10.4
 546
 181
 no BLAST hits
MAP0853
 4.04
 660
 219
 no BLAST hits
MAP0855
 0.34
 926
 308/314
 no BLAST hits
MAP0857c
 0.70
 318
 105
 hypothetical protein
MAP0858
 0.44
 537
 178/182
 no BLAST hits
MAP0859c
 0.66
 609
 202
 Mycobacterium phage L5
MAP0860c
 0.04
 885
 294/296
 no BLAST hits
MAP0861
 0.43
 342
 113
 no BLAST hits
MAP0862
 0.48
 989
 329/360
 no BLAST hits
MAP0863
 0.66
 675
 224
 no BLAST hits
MAP0864
 7.42
 426
 141
 no BLAST hits
MAP0865
 0.08
 1272
 423
 Hypothetical 30.9 kDa protein
MAP0866
 3.07
 804
 267
 integrase
MAP0904
 0.81
 371
 124/246
 conserved hypothetical protein
MAP0961c
 0.18
 719
 239/352
 hypothetical protein
MAP1087
 0.43
 441
 146
 prob. peptide transp. system permease, N-terminal
MAP1121c
 0.32
 498
 165
 putative substrate binding protein
MAP1174c
 1.64
 756
 251
 glucose-6-phosphate 1-dehydrogenase
MAP1204
 2.61
 735
 244
 putative exported p60 protein homologue
MAP1233
 0.04
 723
 240
 conserved hypothetical protein
MAP1345
 0.69
 600
 199
 no BLAST hits
MAP1388
 0.08
 396
 131
 no BLAST hits
MAP1416c
 0.45
 666
 221
 no BLAST hits
MAP1417c
 0.54
 435
 144
 no BLAST hits
MAP1609c
 0.05
 987
 328/330
 antigen 85B, mycolyltransferase
MAP1636c
 1.12
 425
 141/157
 no BLAST hits
MAP1643
 0.45
 2277
 759/762
 isocitrate lyase, [beta] module
MAP1655c
 0.93
 381
 126
 hypothetical protein
MAP1730c
 0.26
 1023
 340
 putative ATP/GTP-binding protein
MAP1931c
 0.32
 1104
 367
 anthranilate phosphoribosyltransferase
MAP2077c
 1.66
 330
 109
 hypothetical protein
MAP2116c
 0.27
 1269
 422
 cell invasion protein
MAP2121c
 7.14
 887
 295/307
 major membrane protein I
MAP2121c-his
 0.99
 924
 307
 major membrane protein I
MAP2151
 2.79
 438
 145
 no BLAST hits
MAP2155
 9.57
 312
 103
 similar to IS6110
MAP2156
 0.59
 963
 320
 putative transposase GEN: X52471)
MAP2157
 0.38
 1221
 406
 IS900
MAP2158
 2.40
 582
 193
 no BLAST hits
MAP2182c
 0.26
 435
 144
 conserved hypothetical protein
MAP2231
 0.31
 1422
 473
 PKS-associated protein, unknown function
MAP2360c
 0.13
 534
 177
 hypothetical protein
MAP2380
 2.00
 600
 200/550
 acyl-CoA synthase
MAP2657
 0.26
 651
 216
 putative oxidoreductase
MAP2663c
 0.03
 510
 169
 putative integral membrane protein
MAP2676c
 1.30
 354
 117
 hypothetical protein
MAP2734
 0.37
 1341
 446
 putative dioxygenasesdiooxygenases
MAP2740
 0.24
 666
 221
 putative membrane protein
MAP2751
 0.70
 582
 193
 no BLAST hits
MAP2753
 0.17
 309
 103/252
 hypothetical protein
MAP2761c
 0.03
 692
 230/238
 no BLAST hits
MAP2762c
 1.45
 425
 141/146
 no BLAST hits
MAP2764c
 0.10
 290
 96/149
 no BLAST hits
MAP2765c
 0.06
 539
 179/396
 no BLAST hits
MAP2767c
 0.42
 473
 157/183
 no BLAST hits
MAP2963c
 0.88
 1499
 499/874
 no BLAST hits
MAP3084c-his
 1.0
 684
 227
 probable secreted protein
MAP3121
 0.38
 855
 284
 enoyl-CoA hydratase/isomerase superfamily
MAP3129
 0.09
 404
 134/141
 hypothetical protein
MAP3131
 0.30
 1096
 365/942
 conserved large membrane protein
MAP3155c
 1.22
 273
 90
 hypothetical protein
MAP3434
 0.18
 690
 230/330
 possible membrane protein
MAP3437c
 0.29
 843
 279/280
 no BLAST hits
MAP3531c
 0.28
 1034
 344/352
 antigen 85C, mycolytransferase
MAP3734c
 0.19
 1559
 519/593
 putative ABC transporter ATP-binding protein
MAP3735c
 0.42
 1181
 393/429
 part of heavy metal tolerance protein
MAP3743
 0.85
 998
 332/348
 hypothetical protein
MAP3751
 0.11
 879
 293/979
 conserved large membrane protein
MAP3753
 0.64
 1353
 450
 hypothetical protein
MAP3761c
 0.07
 729
 242
 conserved membrane protein
MAP3771
 0.02
 294
 97
 50S ribosomal protein L31
MAP3817c
 0.65
 939
 312
 no BLAST hits
MAP3833c
 0.13
 626
 208/260
 hypothetical protein
MAP3840
 0.10
 1872
 623
 70 kD heat shock protein, chromosome replication
MAP3902c
 0.15
 522
 173
 Serine/threonine kinase (EC 2.7.1.37) [Myc...
MAP3903c
 0.42
 531
 176
 Serine/threonine kinase (EC 2.7.1.37) [Myc...
MAP3954
 0.05
 279
 92
 conserved hypothetical protein
MAP4014
 0.21
 951
 316
 probable 4-amino butyrate transporter, N-terminal
MAP4025
 0.32
 854
 284/325
 cytochrome c-type biogenesis protein
MAP4129
 0.26
 965
 321/336
 ABC transporter
MAP4198
 0.13
 1283
 427/441
 SecY subunit of preprotein translocase
MAP4207c
 5.28
 686
 228/231
 probable ATP-binding transport protein
MAP4199
 0.03
 540/546
 179/181
 probable adenylate kinase
MAP4228
 0.05
 222
 73
 initiation factor IF-1

aProtein name corresponds to the genome project designation (Li et al., 2005). A second designation is included if known.

bProtein concentration is reported in as measured by Nanodrop spectrometry at 280nm.

cGene size is reported in base pairs (bp). In cases where only a portion of the M. avium subsp paratuberculosis gene was cloned, the number of bp present in the clone is reported first followed by the number of bp in the full-length M. avium subsp paratuberculosis gene.

dNumber of M. avium subsp paratuberculosis amino acids present in the fusion protein. In cases where only a portion of the M. avium subsp paratuberculosis protein is represented, the number of amino acids present is listed first followed by the total number of M. avium subsp paratuberculosis amino acids in the full-length protein.

eNA is not applicable.

Bannantine et al. Proteome Science 2008 6:5   doi:10.1186/1477-5956-6-5