Table 3

Yield of soluble recombinant protein. Results sorted according to ORF size [kDa].
ORF size [kDa]
 ORF count [n]
 purif. ORF [n]
 Yield ORF [%]
 Hisa Hisb
 NusAa Hisb
 GSTa GSTb
 MBPa MBPb
 MBPa Hisb
0–9
 2
 1
 50
 1
 0
 0
 0
 1
10–19
 10
 9
 90
 4
 8
 8
 5
 7
20–29
 21
 15
 70
 6
 11
 8
 6
 14
30–39
 15
 10
 70
 2
 8
 4
 3
 6
40–49
 15
 10
 70
 3
 10
 6
 4
 7
50–59
 3
 1
 30
 0
 1
 0
 0
 0
60–69
 8
 3
 40
 2
 1
 0
 0
 0
70–79
 5
 2
 40
 1
 1
 0
 0
 0
80–89
 7
 2
 30
 0
 2
 0
 0
 1
90–99
 6
 0
 0
 0
 0
 0
 0
 0
≥ 100
 4
 0
 0
 0
 0
 0
 0
 0

a fusion tag

b tag employed for purification strategy

Kohl et al. Proteome Science 2008 6:4   doi:10.1186/1477-5956-6-4