|
Differentially expressed proteins identified by proteomics from retinas of ED7, ED11 and ED15 of embryo chick retina. |
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| Theoretical value |
Experimental value |
Changes(ratio) |
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| Protein |
Est'Z |
Taxonomy |
Sequence coverage(%) |
ncbi ID |
pH |
Mr (kDa) |
pH |
Mr (kDa) |
ED7:ED7:ED15 |
|
|
|
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| S1 |
Fatty acid-binding protein, retina |
2.13 |
Chick |
61 |
Q05423 |
5.6 |
15.02 |
5.5 |
15 |
(-):(+):(+) |
| S2 |
Wnt-9 |
0.98 |
P. hagfish |
37 |
P28124 |
11.3 |
15.06 |
5.75 |
10 |
1:14:4.6 |
| S3 |
Ubiquitin like protein, SMT3A |
0.4 |
Human |
24 |
P55854 |
5.3 |
11.68 |
7 |
10 |
1:9.6:1.2 |
| S4 |
unnamed protein product |
0.66 |
Mouse |
12 |
BAC32207.1 |
5.3 |
80.05 |
6.75 |
70 |
1:2.4:2.3 |
| S5 |
Alpha enolase |
2.38 |
Chick |
35 |
P51913 |
6.2 |
47.63 |
6.5 |
100 |
1:2.2:1.7 |
| S6 |
Sin3B |
0.76 |
Human |
10 |
AAH05113 |
6.6 |
49.62 |
9 |
45 |
2.1:1:0.3 |
| S7 |
hypothetical protein |
1.03 |
Human |
23 |
CAD38885.1 |
9.2 |
26.71 |
9 |
35 |
2.5:1:1.7 |
| S8 |
unnnamed protein product |
0.99 |
Human |
44 |
BAB14762.1 |
5.5 |
29.35 |
4 |
20 |
2.4:1:1.7 |
| S9 |
unnamed protein product |
1.51 |
Human |
43 |
BAB85061.1 |
5.4 |
17.7 |
4 |
20 |
4.2:1:1.6 |
| S10 |
ZZ type zinc finger containing protein |
0.52 |
C. elegans |
15 |
NP498699.1 |
4.5 |
64.85 |
4.5 |
30 |
7.5:1:4.2 |
| S11 |
GABA receptor-A beta-3 |
0.61 |
Chick |
12 |
P19019 |
9.4 |
54.76 |
5 |
45 |
2.8:1:4.1 |
| S12 |
Flice |
0.72 |
Mouse |
18 |
CAA04196.1 |
5.3 |
49.35 |
5 |
70 |
1:2.9:(-) |
| S13 |
Keratin-9 |
2.43 |
Human |
36 |
NP000217.1 |
5.1 |
62.2 |
9 |
45 |
1:2.3:4.0 |
|
S1 – S13 means ID numbers of spots. Taxonomy category was used for search. Probability, Est'Z, sequence coverage, and theoretical value of pI and Mr were obtained from the Pro Found search. The calculation of experimental pI and Mr was based on the migration of the protein in a 2D gel. Changes indicate the ED7 chick retinal protein volume ratio compared with that of ED11 and ED15. (-) indicate that protein spots were not detected | ||||||||||
Mizukami et al. Proteome Science 2008 6:3 doi:10.1186/1477-5956-6-3 |
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