Proteome profiling of embryo chick retina

Mina Mizukami¹ , Takashi Kanamoto¹ , Nazariy Souchelnytskyi² and Yoshiaki Kiuchi¹

¹Department of Ophthalmology and Visual Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan

²Karolinska Biomics Center, Dept. Oncology Pathology Karolinska University Hospital, Stockholm, Sweden

author email corresponding author email

Proteome Science 2008, 6:3doi:10.1186/1477-5956-6-3


Published:	22 January 2008

Abstract

Background

Little is known regarding the molecular pathways that underlie the process of retinal development. The purpose of this study was to identify proteins which may be involved in development of retina. We used a proteomics-based approach to identify proteins that are up- or down-regulated during the development of the embryo chick retina.

Results

Two-dimensional gel electrophoresis was performed with the retina of embryo chicken, which was obtained from embryos of day 7 (ED7) and of day 11 (ED11). The protein spots showing significant differences were selected for identification by MALDI mass spectrometry. Thirteen proteins were differentially expressed; seven proteins were up-regulated in embryo retina of chicken at ED 11 and six proteins were down-regulated. Significant proteins were also evaluated in embryo day 15 (ED15). Some of identified proteins were known to regulate cell proliferation, cell death, transport, metabolism, organization and extracellular matrix, and others also included novel proteins.

Conclusion

We identified thirteen proteins which differentially expressed in embryonal retina of chicken at day 7, as compared to the retina of embryo of day 11. They were various regulatory proteins for cellular signaling.