A simple and reliable protocol for mouse serum proteome profiling studies by use of two-dimensional electrophoresis and MALDI TOF/TOF mass spectrometry

doi:10.1186/1477-5956-6-25

Proteome Science

Volume 6

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A simple and reliable protocol for mouse serum proteome profiling studies by use of two-dimensional electrophoresis and MALDI TOF/TOF mass spectrometry

Maria Stella Ritorto¹ and Jürgen Borlak¹^,2

¹Department of Drug Research and Medical Biotechnology, Fraunhofer Institute of Toxicology and Experimental Medicine, Nikolai-Fuchs-Str 1, 30625, Hanover, Germany

²Centre for Pharmacology and Toxicology, Hanover Medical School, Carl-Neuberg-Str 1, 30625, Hanover, Germany

author email corresponding author email

Proteome Science 2008, 6:25doi:10.1186/1477-5956-6-25


Published:	12 September 2008

Additional files

Additional file 1:

Validation of the new protein entries. We analysed the same spectra with Mascot search engine in ProteinScape™) and PeptideMap (PROWL free softwares). The ionized tryptic peptides resulted matched to the same protein sequences.

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Additional file 2:

Mascot and PeptideMap. Single entries from both Mascot and PeptideMap for the new identifications.

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Additional file 3:

Protein sequences. We listed all identified protein sequence, the PMF (MS analysis) and PFF (tandem-MS analysis). We chose representative tandem MS for every ion peak fitting with the protein sequence.

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