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Open Access Research

Profiling of serum and tissue high abundance acute-phase proteins of patients with epithelial and germ line ovarian carcinoma

Yeng Chen1, Boon-Kiong Lim2, Suat-Cheng Peh3, Puteri Shafinaz Abdul-Rahman1 and Onn Haji Hashim1*

Author Affiliations

1 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

2 Department of Obstetrics & Gynecology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

3 Department of Pathology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

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Proteome Science 2008, 6:20  doi:10.1186/1477-5956-6-20

Published: 18 July 2008

Abstract

Background

Acute-phase response involves the simultaneous altered expression of serum proteins in association to inflammation, infection, injury or malignancy. Studies of the acute-phase response usually involve determination of the levels of individual acute-phase serum proteins. In the present study, the acute-phase response of patients with epithelial (EOCa) and germ-line (GOCa) ovarian carcinoma was investigated using the gel-based proteomic approach, a technique which allowed the simultaneous assessment of the levels of the acute-phase serum high abundance proteins. Data obtained were validated using ELISA and immunostaining of biopsy samples.

Results

Enhanced expression of clusterin (CLU), α1-antitrypsin, haptoglobin and leucine rich glycoprotein was detected in all patients. However, the levels of α1-antichymotrypsin (ACT) was only enhanced in EOCa patients, while patients with GOCa were typically characterized by elevated levels of ceruloplasmin but lower levels of α2-HS glycoprotein. The enhanced expression of CLU in EOCa and GOCa patients and up-regulated expression of ACT specifically in EOCa patients were confirmed by ELISA. Immunohistochemical staining of biopsy samples of EOCa and GOCa patients demonstrated correlation of the acute-phase protein expression.

Conclusion

Patients with EOCa and GOCa demonstrated distinctive aberrant expression of serum and tissue high abundance acute-phase proteins compared to negative control women.