Figure 4.

Immunofluorescence staining of the human melanoma cells using tMeK-specific antibody. Cells treated with or without TSA (200 ng/ml) for 16 h were fixed with formaldehyde and permeabilized with 1% triton X-100. The cells were stained with 10 μg/ml anti-tMeK-FITC antibody with and without the presence of free tMeK (10 μg/ml). The image was captured with the Zeiss fluorescence microscope equipped with Northern Eclipse imaging software with an exposure time of 100 ms. The picture shows the representative images of the staining.