Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53

Bent Honoré¹ , Søren Buus² and Mogens H Claësson²

¹Institute of Medical Biochemistry, University of Aarhus, Ole Worms Allé, Bldg. 1170, DK-8000 Aarhus C, Denmark

²Laboratory of Experimental Immunology, Faculty of Health Sciences, University of Copenhagen, Denmark

author email corresponding author email

Proteome Science 2008, 6:18doi:10.1186/1477-5956-6-18


Published:	10 June 2008

Abstract

Background

Knockout mice with a deletion of p53 spontaneously develop thymic lymphomas. Two cell lines (SM5 and SM7), established from two independent tumours, exhibited about fifty to seventy two-fold differentially expressed proteins compared to wild type thymocytes by two-dimensional gel electrophoresis (2D-PAGE).

Results

Protein spots excised from 2D-PAGE gels, were subjected to in-gel tryptic digestion and identified by liquid chromatography – tandem mass spectrometry. A total of 47 protein spots were identified. Immunological verification was performed for several of the differentially regulated proteins where suitable antibodies could be obtained. Functional annotation clustering revealed similarities as well as differences between the tumours. Twelve proteins that changed similarly in both tumours included up-regulation of rho GDP-dissociation inhibitor 2, proteasome subunit α type 3, transforming acidic coiled-coil containing protein 3, mitochondrial ornithine aminotransferase and epidermal fatty acid binding protein and down-regulation of adenylosuccinate synthetase, tubulin β-3 chain, a 25 kDa actin fragment, proteasome subunit β type 9, cofilin-1 and glia maturation factor γ.

Conclusion

Some of the commonly differentially expressed proteins are also differentially expressed in other tumours and may be putative diagnostic and/or prognostic markers for lymphomas.