 ResearchProteome of monocyte priming by lipopolysaccharide, including changes in interleukin-1beta and leukocyte elastase inhibitorMichael J Pabst1 1 Dental Research Center and Department of Periodontology, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN 38163, USA 2 Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN 38163, USA 3 Department of Neurology, and the Charles B. Stout Mass Spectrometry Laboratory, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
Proteome Science 2008, 6:13doi:10.1186/1477-5956-6-13
Additional filesAdditional File 1: 2D gel image from unprimed monocytes (No LPS). 2D gel from monocytes incubated for 16 h with no addition. Stained with Sypro ruby. Spots (or the positions of missing spots) are located just to the left of the identifying letters. Format: PDF Size: 2.2MB Download file This file can be viewed with: Adobe Acrobat Reader Additional File 2: 2D gel image from monocytes primed with LPS. 2D gel from monocytes exposed to LPS (2 ng/ml) for 16 h. Format: PDF Size: 2MB Download file This file can be viewed with: Adobe Acrobat Reader Additional File 3: 2D gel image from unprimed monocytes treated with AEBSF. 2D gel from monocytes exposed to AEBSF (250 μM) for 16 h in the absence of LPS. Format: PDF Size: 2.2MB Download file This file can be viewed with: Adobe Acrobat Reader Additional File 4: 2D gel image from monocytes primed with LPS and treated with AEBSF. 2D gel from monocytes exposed to AEBSF (250 μM) for 16 h in the presence of LPS. Format: PDF Size: 2.3MB Download file This file can be viewed with: Adobe Acrobat Reader Additional File 5: Histograms of 30 differentially expressed protein spots in 2D gels from monocytes treated ± LPS ± AEBSF. The first bar is Time 0, a control from monocytes taken at the start of culture. The second bar is No LPS for 16 h, third bar is LPS for 16 h, fourth bar is AEBSF alone for 16 h, and fifth bar is LPS + AEBSF for 16 h. The sixth bar shows the effect of AEBSF added at the end of culture at 16 h to monocytes not treated with LPS, and the seventh bar shows AEBSF added at the end of culture to monocytes exposed to LPS for 16 h. Four patterns of expression were observed: yellow background indicates proteins whose expression increased in response to AEBSF, pink indicates proteins that decreased with AEBSF, blue indicates proteins that decreased with LPS, and green indicates proteins that increased with LPS. Format: PDF Size: 52KB Download file This file can be viewed with: Adobe Acrobat Reader |
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