Table 2

Cytosolic differentially expressed proteins identification and regulation




hTERTt-WI38/WI38
 hTERT-WI38/HPV-WI38
 Reference




Swiss-Prot AC
 Gene name, STRING protein association network legend
 Protein name
 Function
 T-test
 Regulation
 T-test
 Regulation
[O43852]
 CALR
 Calumenin precursor
 Ca(2+)-binding, secretory pathway
 1.0E-03
 2.27
 1.6E-02
 1.5
 [40]
[Q15293]
 RCN1
 Reticulocalbin-1 precursor
 Ca(2+)-binding, secretory pathway
 1.3E-04
 2.11
 4.2E-04
 1.85
 [40]
[P14625]
 HSP90B1
 Endoplasmin precursor (GRP94)
 ER chaperone, Ca(2+) binding, anti-apoptosis
 1.4E-05
 2.1
 1.6E-03
 1.26
 [47, 59]
[Q14697]
 GANAB
 Neutral alpha-glucosidase AB precursor
 Protein O-glucosyl hydrolase
 4.9E-08
 2.06
 5.5E-06
 1.42
[O95302]
 FKBP9L
 FK506-binding protein 9 precursor (FKBP9)
 Peptidyp propyl isomerase (PPI), accelerate protein folding
 9.9E-05
 2.04
 3.9E-03
 1.41
 [52]
[Q9Y4L1]
 HYOU1
 150 kDa oxygen-regulated protein precursor (ORP150)
 ER chaperone, exchange factor for GRP78
 1.2E-03
 1.98
 2.3E-03
 1.8
 [47, 60]
[Q9BS26]
 TXNDC4
 Thioredoxin domain-containing protein 4 precursor (ERp44)
 Control of oxidating protein folding, ERO1L partner
 3.5E-06
 1.89
 7.2E-05
 1.57
 [61, 62]
[O43852]
 CALU
 Calumenin precursor
 Ca(2+)-binding, secretory pathway
 1.80E-05
 1.80
 3.7E-03
 1.35
 [40]
[Q15293]
 RCN1
 Reticulocalbin-1 precursor
 Ca(2+)-binding, secretory pathway
 2.5E-02
 1.78
 6.2E-02
 1.51
 [40]
[O75131]
 CPNE3
 Copine-3
 Ca(2+)-dependent phospholipid-binding proteins
 7.4E-04
 1.73
 1.2E-03
 1.59
 [63, 64]
[Q8NBS9]
 TXNDC5
 Thioredoxin domain-containing protein 5 precursor (Erp46)
 Thiol oxydoreductase
 3.2E-03
 1.73
 4.9E-03
 1.65
 [62, 65]
[P11021]
 HSPA5
 78 kDa glucose-regulated protein precursor (GRP78)
 ER chaperone, Ca(2+)-binding, ER stress sensor, anti-apoptosis
 1.5E-02
 1.65
 7.4E-03
 1.57
 [47, 66]
[P13674]
 P4HA1
 Prolyl 4-hydroxylase alpha-1 subunit precursor
 Hypoxia-inducible collagen synthesis
 2.0E-08
 1.65
 1.3E-06
 1.77
 [67]
[O15460]
 P4HA2
 Prolyl 4-hydroxylase alpha-2 subunit precursor
 Hypoxia-inducible collagen synthesis
 2.8E-04
 1.64
 1.5E-03
 1.43
 [67]
[P07237]
 P4HB
 Protein disulfide-isomerase precursor
 Rearrangement of -S-S-bonds in proteins
 2.0E-06
 1.64
 2.1E-04
 1.28
 [68]
[Q9H2D6]
 TRIOBP
 TRIO and F-actin-binding protein
 May regulate actin cytoskeletal organization
 8.0E-05
 1.59
 4.9E-03
 1.35
[P30101]
 GRP58
 Protein disulfide-isomerase A3 precursor (PDI-A3)
 Rearrangement of -S-S-bonds in proteins
 8.6E-07
 1.57
 2.8E-06
 1.48
 [68]
[Q96HE7]
 ERO1L
 ERO1-like protein alpha precursor (ERO1L)
 Protein disulfide isomerases oxydoreductase
 2.7E-02
 1.54
 4.7E-02
 1.44
 [61, 69]
[P27797]
 CALR
 Calreticulin precursor
 ER chaperone, Ca(2+)-binding, glycoprotein folding
 2.9E-02
 1.46
 7.8E-03
 1.61
 [47]
[Q15084]
 PDIA6
 Protein disulfide-isomerase A6 precursor (PDI-A6)
 Rearrangement of -S-S-bonds in proteins
 8.8E-05
 1.45
 1.1E-04
 1.31
 [68]
[Q99439]
 CNN2
 Calponin-2
 Ca(2+)-binding, actin-binding RNA helicase which
 2.1E-04
 1.33
 2.7E-03
 1.25
 [70, 71]
[P60842]
 EIF4A1
 Eukaryotic initiation factor 4A-I
 mediates binding of mRNA to the ribosome
 6.6E-05
 1.33
 8.6E-05
 1.48
[P30101]
 GRP58
 Protein disulfide-isomerase A3 precursor (PDI-A3)
 Rearrangement of -S-S-bonds in proteins
 3.9E-03
 1.29
 2.0E-03
 1.44
 [68]
[P27797]
 CALR
 Calreticulin precursor
 ER chaperone, Ca(2+)-binding, glycoprotein folding
 0.18
 1.26
 2.6E-02
 1.68
 [47]
[P51665]
 PSMD7
 26S proteasome non-ATPase regulatory subunit 7
 Regulation of ubiquitinated proteins degradation
 2.2E-02
 0.76
 1.4E-02
 0.71
[P04406]
 HSD35
 Glyceraldehyde-3-phosphate dehydrogenase
 Glycolytic pathway
 1.1E-02
 0.75
 7.7E-04
 0.70
[Q99426]
 CKAP1
 Tubulin-specific chaperone B
 Chaperone, tubulin organisation
 1.3E-03
 0.75
 8.8E-04
 0.72
[P04406]
 HSD35
 Glyceraldehyde-3-phosphate dehydrogenase
 Glycolytic pathway
 4.9E-03
 0.74
 9.4E-03
 0.76
[P09211]
 GSTP1
 Glutathione S-transferase P
 Oxidative stress
 6.1E-03
 0.72
 4.7E-02
 0.81
[P04075]
 ALDOA
 Fructose-bisphosphate aldolase A
 Glycolytic pathway
 6.4E-03
 0.71
 7.2E-02
 0.85
[P04075]
 ALDOA
 Fructose-bisphosphate aldolase A
 Glycolytic pathway
 8.2E-04
 0.69
 4.7E-02
 0.75
[P04406]
 HSD35
 Glyceraldehyde-3-phosphate dehydrogenase
 Glycolytic pathway
 1.2E-03
 0.61
 1.3E-03
 0.69
[P04792]
 HSPB1
 Heat-shock protein beta-1 (HSP27)
 Chaperone, actin organization
 3.8E-03
 0.61
 3.4E-03
 0.65
[P05120]
 SERPINB2
 Plasminogen activator inhibitor 2 precursor
 Plasminogen activator inhibitor
 3.3E-04
 0.56
 1.3E-03
 0.59
[P17987]
 TCP1
 T-complex protein 1 subunit alpha
 Chaperone, protein folding, actin and tubulin folding
 1.7E-02
 0.56
 3.7E-03
 0.67
[P08758]
 ANXA5
 Annexin A5
 Ca(2+)-dependant phospholipids-binding, apoptosis regulation
 2.1E-04
 0.39
 2.6E-04
 0.40
[P07737]
 PFN1
 Profilin-1
 Intranuclear movements, assembly of transcription complexes
 3.3E-04
 0.38
 1.0E-02
 0.33
[P07737]
 PFN1
 Profilin-1
 Intranuclear movements, assembly of transcription complexes
 3.2E-02
 0.37
 2.7E-02
 0.4

List of the proteins differentially expressed due to hTERT transfection in the cytosolic fraction of human WI38 cells. Listed by decreasing regulation.

Mazzucchelli et al. Proteome Science 2008 6:12   doi:10.1186/1477-5956-6-12