Increase in local protein concentration by field-inversion gel electrophoresis

doi:10.1186/1477-5956-5-18

Proteome Science

Volume 5

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Increase in local protein concentration by field-inversion gel electrophoresis

Henghang Tsai¹ , Teck Yew Low¹ , Steve Freeby² , Aran Paulus² , Kalpana Ramnarayanan¹ , Chung-pui Paul Cheng¹ and Hon-chiu Eastwood Leung³

¹Medical Proteomics and Bioanalysis Section, Genome Institute of Singapore, Singapore

²Bio-Rad Laboratories, Hercules, USA

³Department of Molecular and Cellular Biology, Department of Pediatrics, Baylor College of Medicine and Texas Children's Hospital, Houston, USA

author email corresponding author email

Proteome Science 2007, 5:18doi:10.1186/1477-5956-5-18


Published:	26 September 2007

Additional files

Additional file 1:

The physical setup of the field-inversion device. Figure S1 shows the links of the pulsed-inversion device from the power pack to the gel tank.

Format: PDF Size: 81KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 2:

The timer screen when the pulsed-inversion device was set at a pulse frequency of ta/tr = 80/40 msec. A movie showes the real-time readout of the changes of voltage output on the timer of the pulsed-inversion device.

Format: ASF Size: 98KB Download file

Additional file 3:

The oscilloscope screen when the pulsed-inversion device was set at a pulse frequency of ta/tr = 80/40 msec. Description: A movie shows the real-time readout from the Tektronix oscilloscope model TDS 1000 when the pulsed-inversion device was set at a ta/tr = 80/40 msec.

Format: ASF Size: 362KB Download file

Additional file 4:

The timer screen when the pulsed-inversion device was set at a pulse frequency of ta/tr = 160/40 msec. A movie showes the real-time readout of the changes of voltage output on the timer of the pulsed-inversion device when the device was set at a ta/tr = 160/40 msec.

Format: ASF Size: 128KB Download file

Additional file 5:

The oscilloscope screen when the pulsed-inversion device was set at a pulse frequency of ta/tr = 160/40 msec. A movie shows the real-time readout from the Tektronix oscilloscope model TDS 1000 when the pulsed-inversion device was set at a ta/tr = 160/40 msec.

Format: ASF Size: 220KB Download file