|
Non-PM proteins identified in the lipid rafts of neonatal mouse brain |
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| Acc. No. |
Protein name |
Pepa |
% Covb |
MW (KDa) |
Loc.c |
Spectral countd |
SDe |
|
|
|||||||
| P69893 |
Tubulin beta-1 chain |
12 |
38.60 |
49.67 |
cyto |
262.0 |
14.0 |
| P68361 |
Tubulin alpha-1 chain |
9 |
30.44 |
50.15 |
cyto |
175.0 |
20.0 |
| Q71FK5 |
Actin, cytoplasmic 1 (Beta-actin) |
5 |
21.39 |
41.74 |
cyto |
102.5 |
4.5 |
| Q03265 |
ATP synthase alpha chain |
10 |
26.63 |
59.75 |
mc |
63.0 |
4.0 |
| P56480 |
ATP synthase beta chain |
9 |
20.83 |
56.30 |
mc |
51.5 |
5.5 |
| P04104 |
Keratin, type II cytoskeletal 1 |
3 |
5.59 |
65.1 |
cyto |
37.0 |
4.0 |
| P62629 |
Elongation factor 1-alpha 1 |
3 |
9.33 |
50.11 |
cyto |
36.0 |
5.0 |
| P19378 |
Heat shock cognate 71 kDa protein |
4 |
10.39 |
70.8 |
cyto |
33.0 |
1.0 |
| Q922U2 |
Keratin, type II cytoskeletal 5 |
2 |
4.15 |
61.8 |
cyto |
26.5 |
6.5 |
| P14873 |
Microtubule-associated protein 1B (MAP 1B) |
7 |
4.34 |
270.41 |
cyto |
25.5 |
3.5 |
| Q6IFZ6 |
Keratin, type II cytoskeletal 1b |
2 |
4.20 |
61.4 |
cyto |
25.5 |
3.5 |
| P97427 |
Dihydropyrimidinase-related protein 1 |
4 |
8.93 |
62.17 |
cyto |
25.0 |
0.0 |
| Q62188 |
Dihydropyrimidinase-related protein 3 |
7 |
20.39 |
61.94 |
cyto |
21.5 |
0.5 |
| P14733 |
Lamin-B1 |
5 |
12.80 |
66.66 |
nuc |
19.0 |
3.0 |
| P68372 |
Tubulin beta-2C chain |
2 |
6.76 |
49.83 |
cyto |
18.5 |
1.5 |
| Q6IG00 |
Keratin, type II cytoskeletal 4 |
2 |
1.68 |
57.7 |
cyto |
18.0 |
1.0 |
| Q60932 |
Voltage-dependent anion-selective channel protein 1 |
7 |
38.31 |
32.35 |
mc |
16.5 |
3.5 |
| P48962 |
ADP/ATP translocase 1 |
3 |
11.15 |
32.77 |
mc |
15.0 |
2.0 |
| Q922F4 |
Tubulin beta-6 chain |
2 |
3.81 |
50.09 |
cyto |
14.5 |
0.5 |
| Q04447 |
Creatine kinase B-type |
3 |
12.63 |
42.71 |
cyto |
13.5 |
2.5 |
| P67778 |
Prohibitin |
4 |
21.03 |
29.82 |
mc |
12.5 |
1.5 |
| Q10758 |
Keratin, type II cytoskeletal 8 |
2 |
3.33 |
53.9 |
cyto |
12.5 |
0.5 |
| Q61696 |
Heat shock 70 kDa protein 1A |
2 |
5.78 |
70.08 |
cyto |
12.0 |
1.0 |
| O08553 |
Dihydropyrimidinase-related protein 2 |
5 |
13.84 |
62.17 |
cyto |
11.5 |
2.5 |
| P46633 |
Heat shock protein HSP 90-alpha (HSP 86) |
2 |
3.56 |
84.72 |
cyto |
10.5 |
2.5 |
| P63101 |
14-3-3 protein zeta/delta |
2 |
12.30 |
27.77 |
cyto |
10.5 |
0.5 |
| Q60930 |
Voltage-dependent anion-selective channel protein 2 |
3 |
14.29 |
31.73 |
mc |
10.0 |
0.0 |
| Q9ERD7 |
Tubulin beta-3 chain |
2 |
9.35 |
50.42 |
cyto |
10.0 |
1.0 |
| P50672 |
Cytochrome c oxidase subunit 2 |
2 |
11.50 |
25.82 |
mc |
9.0 |
0.0 |
| Q9DCT2 |
NADH-ubiquinone oxidoreductase 30 kDa subunit |
2 |
6.35 |
34.00 |
mc |
8.5 |
0.5 |
| P18760 |
Cofilin-1 (Cofilin, non-muscle isoform) |
2 |
15.24 |
18.43 |
nuc |
8.5 |
1.5 |
| P07823 |
78 kDa glucose-regulated protein |
5 |
11.18 |
72.38 |
er |
8.5 |
1.5 |
| P11497 |
Acetyl-CoA carboxylase 1 |
1 |
4.24 |
37.62 |
cyto |
8.0 |
1.0 |
| Q60931 |
Voltage-dependent anion-selective channel protein 3 |
3 |
13.83 |
30.75 |
mc |
7.5 |
0.5 |
| P09445 |
Elongation factor 2 |
2 |
2.92 |
95.27 |
cyto |
7.5 |
0.5 |
| P62977 |
Ubiquitin |
1 |
21.33 |
8.57 |
cyto |
7.0 |
1.0 |
| P19783 |
Cytochrome c oxidase subunit IV isoform 1 |
1 |
7.14 |
19.53 |
mc |
7.0 |
2.0 |
| P14152 |
Malate dehydrogenase |
1 |
3.61 |
36.35 |
mc |
6.5 |
0.5 |
| P11499 |
Heat shock protein HSP 90-beta |
2 |
3.88 |
83.20 |
cyto |
6.5 |
0.5 |
| P12787 |
Cytochrome c oxidase polypeptide Va |
1 |
10.42 |
16.03 |
mc |
6.5 |
0.5 |
| P31253 |
Ubiquitin-activating enzyme E1 X |
2 |
7.35 |
50.99 |
cyto |
6.0 |
3.0 |
| P35564 |
Calnexin |
2 |
4.92 |
67.28 |
er |
6.0 |
1.0 |
| Q91VD9 |
NADH-ubiquinone oxidoreductase 75 kDa subunit |
2 |
3.99 |
79.75 |
mc |
5.5 |
2.5 |
| P56135 |
ATP synthase f chain, mitochondrial |
2 |
26.74 |
10.21 |
mc |
5.5 |
1.5 |
| P51881 |
ADP/ATP translocase 2 |
2 |
8.11 |
32.80 |
mc |
5.5 |
0.5 |
| Q8R429 |
SR Ca(2+)-ATPase 1 |
2 |
3.12 |
109.43 |
er |
5.0 |
1.0 |
| Q9DB20 |
ATP synthase O subunit |
2 |
9.91 |
23.36 |
mc |
5.0 |
1.0 |
| Q8R429 |
Calcium pump 1 (SERCA1) |
2 |
3.12 |
109.43 |
er |
5.0 |
1.0 |
| Q91V61 |
Sideroflexin-3 |
1 |
4.06 |
35.41 |
mc |
4.5 |
0.5 |
| P03995 |
Glial fibrillary acidic protein, astrocyte (GFAP) |
1 |
2.56 |
49.92 |
cyto |
4.5 |
1.5 |
| Q9CQV8 |
14-3-3 protein beta/alpha |
1 |
5.43 |
21.22 |
cyto |
4.5 |
2.5 |
| P68368 |
Tubulin alpha-4 chain |
2 |
3.11 |
50.14 |
cyto |
3.5 |
0.5 |
| P62962 |
Profilin-1 (Profilin I) |
2 |
21.74 |
14.83 |
cyto |
3.5 |
0.5 |
| Q8QZT1 |
Acetyl-CoA acetyltransferase |
2 |
7.09 |
44.82 |
mc |
3.5 |
0.5 |
| P62962 |
Profilin-1 |
2 |
21.74 |
14.82 |
cyto |
3.5 |
0.5 |
| Q02053 |
Ubiquitin-activating enzyme E1 1 |
3 |
4.82 |
117.81 |
cyto |
3.0 |
1.0 |
| P42932 |
T-complex protein 1 subunit theta |
3 |
6.59 |
59.43 |
cyto |
3.0 |
2.0 |
| O35129 |
Prohibitin-2 |
4 |
21.03 |
29.82 |
mc |
3.0 |
1.0 |
| P31324 |
Prkar2b |
1 |
4.11 |
46.04 |
cyto |
3.0 |
0.0 |
| P20357 |
Microtubule-associated protein 2 (MAP 2) |
2 |
1.20 |
198.98 |
cyto |
3.0 |
0.0 |
| P34926 |
Microtubule-associated protein 1A (MAP 1A) |
2 |
2.36 |
299.53 |
cyto |
3.0 |
0.0 |
| P52480 |
Pyruvate kinase isozyme M2 |
4 |
12.48 |
57.76 |
mc |
3.0 |
2.0 |
| P63209 |
S-phase kinase-associated protein 1A |
1 |
9.32 |
18.53 |
cyto |
3.0 |
1.0 |
| P60879 |
Synaptonemal complex protein 3 |
3 |
6.01 |
27.1 |
nuc |
3.0 |
1.0 |
| O88809 |
Neuronal migration protein doublecortin |
1 |
3.56 |
40.61 |
cyto |
2.5 |
0.5 |
| P17156 |
Heat shock-related 70 kDa protein 2 |
2 |
3.01 |
69.74 |
cyto |
2.5 |
1.5 |
| Q9EQF6 |
Dihydropyrimidinase-related protein 5 |
1 |
3.20 |
61.52 |
cyto |
2.5 |
0.5 |
| Q8BH59 |
Calcium-binding mitochondrial carrier protein Aralar1 |
2 |
4.59 |
74.57 |
mc |
2.5 |
0.5 |
| P48670 |
Vimentin |
1 |
4.25 |
51.85 |
cyto |
2.5 |
1.5 |
| P80315 |
T-complex protein 1 subunit delta |
1 |
2.98 |
57.94 |
cyto |
2.0 |
0.0 |
| P11984 |
T-complex protein 1 subunit alpha A |
1 |
4.14 |
60.34 |
cyto |
2.0 |
0.0 |
| Q9JKK8 |
Serine-protein kinase ATR |
1 |
2.36 |
84.26 |
nuc |
2.0 |
0.0 |
| Q04899 |
Serine/threonine-protein kinase PCTAIRE-3 |
1 |
3.78 |
51.85 |
nuc |
2.0 |
1.0 |
| Q99PT1 |
Rho GDP-dissociation inhibitor 1 (Rho GDI 1) |
1 |
7.88 |
23.41 |
er |
2.0 |
0.0 |
| Q61879 |
Myosin-10 |
1 |
3.09 |
49.59 |
cyto |
2.0 |
0.0 |
| P24638 |
Lysosomal acid phosphatase |
1 |
2.13 |
48.51 |
lysosome |
2.0 |
1.0 |
| O70251 |
Elongation factor 1-beta |
2 |
12.56 |
24.56 |
cyto |
2.0 |
0.0 |
| Q9CPQ8 |
ATP synthase g chain, mitochondrial |
1 |
18.63 |
11.43 |
mc |
2.0 |
0.0 |
| O35627 |
Orphan nuclear receptor NR1I3 |
1 |
2.52 |
40.89 |
nuc |
2.0 |
1.0 |
| P53026 |
60S ribosomal protein L10a |
1 |
6.98 |
24.78 |
nuc |
2.0 |
1.0 |
| P97524 |
Very-long-chain acyl-CoA synthetase |
1 |
2.58 |
70.69 |
er |
1.5 |
0.5 |
| Q01853 |
Transitional endoplasmic reticulum ATPase |
2 |
3.11 |
89.18 |
cyto |
1.5 |
0.5 |
| Q99JR1 |
Sideroflexin-1 |
1 |
5.63 |
35.52 |
mc |
1.5 |
0.5 |
| Q62627 |
PRKC apoptosis WT1 regulator protein |
1 |
4.53 |
35.87 |
nuc |
1.5 |
0.5 |
| Q9DCS9 |
NADH-ubiquinone oxidoreductase PDSW subunit |
1 |
10.92 |
20.89 |
mc |
1.5 |
0.5 |
| P08249 |
Malate dehydrogenase |
2 |
9.79 |
35.60 |
mc |
1.5 |
0.5 |
| Q8BGU5 |
Cyclin fold protein 1 |
1 |
5.00 |
39.39 |
nuc |
1.5 |
0.5 |
| Q8CEE6 |
PAS-kinase (PASKIN) |
1 |
1.16 |
151.27 |
cyto |
1.5 |
0.5 |
| P35980 |
60S ribosomal protein L18 |
1 |
6.99 |
21.5 |
nuc |
1.5 |
0.5 |
|
aPep: peptide counts; b % cov: protein coverage%; c Loc.: subcellular localization. mc. mitochondria, cyto. cytoplasm, er. endoplasmic reticulum; eSpectral count: total MS/MS spectral counts. Number represents mean value of 4 replicates; c standard deviation of spectral counts in 4 replicates. | |||||||
Yu et al. Proteome Science 2007 5:17 doi:10.1186/1477-5956-5-17 |
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