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Proteomic analysis of plasma membrane and secretory vesicles from human neutrophils

Deepa Jethwaney1 email, Md Rafiqul Islam2 email, Kevin G Leidal2 email, Daniel Beltran-Valero de Bernabe3 email, Kevin P Campbell3 email, William M Nauseef2 email and Bradford W Gibson1 email

Buck Institute for Age Research, Novato, CA 94945, USA

Inflammation Program, Department of Medicine, University of Iowa and Veterans Administration Medical Center, Iowa City, IA 52240, USA

Howard Hughes Medical Institute, Senator Paul D. Wellstone Muscular Dystrophy Cooperative Research Center, Department of Molecular Physiology and Biophysics, Department of Neurology, andDepartment of Internal Medicine, University of Iowa, Iowa City, IA 52240, USA

author email corresponding author email

Proteome Science 2007, 5:12doi:10.1186/1477-5956-5-12

Published: 10 August 2007

Additional files

Additional file 1:

Distribution of gp91phox in resting PMN. Figure A1 shows distribution of gp91phox in resting PMN.

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Additional file 2:

Peptides identified by LC-MS/MS. Table A1 gives the list of polymorphonuclear neutrophil (PMN) proteins/peptides identified by mass spectrometry from fractions enriched for (A) secretory vesicles and (B) plasma membrane vesicles.

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Additional file 3:

Spectral counts. Table A2 showing Spectral counts of common protein between fractions enriched for secretory vesicles (SV) and plasma membrane vesicles (PMV).

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