Figure 3.

SDS-PAGE of serum fractions. Proteins of serum fractions containing biomarkers were separated with one-dimensional gel electrophoresis. The two reverse phase beads (RPB) fractions containing 20 and 30 % acetonitrile are shown after staining with colloidal coomassie. Bands in the relevant mass range were excised and eluted. The arrows indicate the bands containing the 9443 biomarker.