Table 1

Dextransucrase (DsrS) production during a high cell density fed-batch cultivation.

Time after induction (h)
Activity of DsrS (U/L)



cell-associated
Secreted



soluble fraction
insoluble fraction


0.5
157
30690
n.d.
1.5
415
71700
n.d.
3.0
365
47422
n.d.

Dextransucrase (DsrS) production during a high cell density fed-batch cultivation with B. megaterium strain WH320 carrying the plasmid pMM1520dsrS after induction with xylose, determined as volumetric activities of DsrS according to an activity staining method previously described [6-7].

n.d.: not detectable

Wang et al. Proteome Science 2006 4:19   doi:10.1186/1477-5956-4-19