Table 1

Identification of several proteins unique to one or more samples. Only those for which a MOWSE score of >65 (p = 0.05)for PMF or >34 (p = 0.05) for MS/MS and for which matching sequences spanned the entire sequence are reported. The experimental pI is the measured pH value for the first dimension fraction. The # Seq. Matched represents the number of fragments matching peptide sequences.
Exp. pI
 RT 2nd Dim. (min)
 Gene Symbol
 NCBI Accession Number
 Sequence MW/pI
 MOWSE Score (PMF)
 Coverage % (# Seq. Matched)
 MOWSE Score (MS/MS)
Cells grown on Matrigel
<4.0
 17.560
 ER60 HSPA5 VIME
 2245365 16507237 37852
 57245/5.88 72431/5.07 53724/5.06
 81 68 86
 29 (13) 23 (11) 28 (13)
 94
<4.0
 18.027
 HSPA5
 16507237
 72431/5.07
 120
 29 (17)
<4.0
 18.777
 HSPA8 HSPA5 ENOA
 24234686 16507237 1167843
 53626/5.62 72431/5.07 47566/7.01
 103 136 96
 25(7) 22(11) 17(8)
 41 36
<4.0
 20.687
 HSP60
 49522865
 61229/5.71
 99
 20(8)
 93
4.63–4.94
 17.167
 ATM
 1497931
 356785/6.37
 74
 9 (19)
7.02–7.32
 16.740
 H2B.1
 1568551
 13928/10.3
 106
 57(9)
 48
7.02–7.32
 16.998
 RPL9 RPS18
 13278765 75517910
 21992/9.96 17708/11.0
 101 73
 53 (8) 38 (9)
 78 44
>8.3
 16.597
 APC
 68533057
 314407/8.03
 70
 9 (18)
>8.3
 16.687
 ALDOA
 4557305
 39964/8.33
 70
 26 (7)
 46
>8.3
 17.094
 GAPDH
 31645
 36244/8.26
 191
 66 (16)
 64
>8.3
 16.634
 RUVBL/CHTF18
 14336725
 130269/9.51
 72
 16 (13)
Cells grown on SISgel
>8.3
 16.774
 RSNL2
 48257203
 60839/9.07
 70
 23 (11)
5.54–5.84
 17.667
 AKR1B1 ATPB
 13529257 28931
 36298/6.82 34026/4.90
 155 96
 49 (14) 25 (6)
 74 59
5.54–5.84
 17.695
 AKR1B1 ATPB
 13529257 28931
 36298/6.82 34026/4.90
 166 90
 48 (17) 28 (7)
 68 79
5.94–6.13
 17.667
 AKR1B1 ATPB
 13529257 28931
 36298/6.82 34026/4.90
 98 48
 55 (13) 22 (3)
 51
Cells grown on plastic
>8.3
 17.407
 GAPDH
 31645
 36244/8.26
 192
 56 (16)
 68

Hurst et al. Proteome Science 2006 4:13   doi:10.1186/1477-5956-4-13