 MethodologyCognate peptide-receptor ligand mapping by directed phage displayThomas Stratmann1,2 1 Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA 2 Universidad de Barcelona, Departamento de Fisiologia, Diagonal 645, 3°, 08028 Barcelona, Spain
Proteome Science 2005, 3:7doi:10.1186/1477-5956-3-7
Additional filesAdditional File 1: Table 1 – Alignment of haemagglutinin amino acids 112–133 with 6 peptide sequences displayed on JC-M13-88 after panning against mAb 12CA5. Six clones of the HA phage display library were analyzed after three rounds of panning with mAb 12CA5. All clones showed a positive reaction in a filter lift using mAb 12CA5. Three clones were identical, and all the clones contained the consensus sequence YPYDVPDYAS against which the mAb is directed (in red bold letters). Format: PDF Size: 5KB Download file This file can be viewed with: Adobe Acrobat Reader Additional File 2: Table 2 – Alignment of haemagglutinin amino acids 157–178 with 15 peptide sequences displayed on JC-M13-88 after panning against the polyclonal rabbit IgG 07431. 15 clones of the HA phage display library were analyzed after three rounds of panning with pAb 07431 raised against the haemagglutinin derived peptide CKRGPDSGFFSRCNWLYKSG. All clones showed a positive reaction in a filter lift using pAb 07431. Several clones were identical and 3 different sequences were identified. All the clones contained the consensus sequence GFFSRLNWLTKS (in blue bold letters). Format: PDF Size: 6KB Download file This file can be viewed with: Adobe Acrobat Reader |
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