Table 1

Set up of the Taguchi optimisation experiment presented in Figure 2 with total number of spots detected in each gel.
Buffer*
 Ampholytes(%)
 CHAPS(%)
 ASB14(%)
 DTT(mM)
 Spot number**
1
 0.5
 0.5
 0.4
 20
 361
2
 0.5
 1.0
 0.8
 40
 339
3
 0.5
 2.0
 1.6
 80
 339
4
 1.0
 0.5
 0.8
 80
 296
5
 1.0
 1.0
 1.6
 20
 351
6
 1.0
 2.0
 0.4
 40
 355
7
 2.0
 0.5
 1.6
 40
 319
8
 2.0
 1.0
 0.4
 80
 327
9
 2.0
 2.0
 0.8
 20
 299

* – all buffers contained 7 M urea, 2 M thiourea, 10 mM Tris

** – number of spots detected, using Phoretix 2D Pro imaging software

Khoudoli et al. Proteome Science 2004 2:6   doi:10.1186/1477-5956-2-6