Optimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach

Guennadi A Khoudoli , Iain M Porter , J Julian Blow and Jason R Swedlow

Division of Gene Regulation and Expression, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK

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Proteome Science 2004, 2:6doi:10.1186/1477-5956-2-6


Published:	9 September 2004

Additional files

Additional File 1:

2DE response to variations of ASB14 and ampholytes concentrations. 50 μg aliquots of total Xenopus egg extract were dissolved in RBs containing 7 M urea, 2 M thiourea, 1.2% CHAPS, 43 DTT and variable amount of ASB14 and ampholytes as indicated on each picture. Spots detected: (A) 682, (B) 227, (C) 662, (D) 640.

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