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Open Access Research

2D-DIGE proteome analysis on the platelet proteins of patients with major depression

Tiao-Lai Huang1*, Mei-Lan Sung1 and Tai-Yuan Chen2*

Author Affiliations

1 Department of Psychiatry, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung, Kaohsiung 833, Taiwan

2 Department of Food Science, Center of Excellence for the Oceans, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 202, Taiwan

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Proteome Science 2014, 12:1  doi:10.1186/1477-5956-12-1

Published: 3 January 2014

Abstract

Introduction

Platelet activation is related to the psychopathology of major depression. We attempted to search and identify protein biomarkers from the platelets of patients with major depression. High resolution two-dimensional Differential Gel Electrophoresis (2D-DIGE), the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), Western blot, and bioinformatic tools were applied to examine the platelet proteins of 10 patients with major depression and 10 healthy controls.

Results

The levels of 8 proteins were significantly different between the patients with major depression in the acute phase and healthy controls. The levels of protein disulfide-isomerase A3 (PDIA3) and F-actin-capping protein subunit beta (CAPZB) were higher in patients with major depression than in healthy controls. The levels of fibrinogen beta chain (FIBB), fibrinogen gamma chain (FIBG), retinoic acid receptor beta (RARB), glutathione peroxidase 1 (GPX1), SH3 domain-containing protein 19 (SH319), and T-complex protein 1 subunit beta (TCPB) were lower in patients with major depression than in healthy controls.

Conclusions

Platelet provided valuable information about the pathways and processes of inflammation/immunity, oxidative stress, and neurogenesis, related to major depression.

Keywords:
2D-DIGE; Western blot; Major depression; Platelet; Fibrinogen