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Open Access Research

Identification of Enolase 1 and Thrombospondin-1 as serum biomarkers in HBV hepatic fibrosis by proteomics

Bin Zhang1, Zi Wang2, Bin Deng3, Xiaoqiong Wu4, Jing Liu2* and Xueping Feng5*

Author Affiliations

1 Department of Histology and Embryology, Xiangya School of Medicine, Central South University, 172 Tongzipo Road, Changsha, Hunan 410013, People’s Republic of China

2 Molecular Biology Research Center, Xiangya School of Medicine, Central South University, 87, Xiangya Road, Changsha, Hunan 410008, People’s Republic of China

3 Department of Cardiology, Xiangya Hospital, Central South University, 87, Xiangya Road, Changsha, Hunan 410008, People’s Republic of China

4 Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, 172 Tongzipo Road, Changsha, Hunan 410013, People’s Republic of China

5 Institute of Medical Sciences, Xiangya Hospital, Central South University, 87, Xiangya Road, Changsha, Hunan 410008, People’s Republic of China

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Proteome Science 2013, 11:30  doi:10.1186/1477-5956-11-30

Published: 11 July 2013

Abstract

Hepatic fibrosis is an inevitable process in the progression of chronic HBV infection to hepatic cirrhosis, but its detailed mechanism is still unknown. Clinic serum biomarkers of HBV hepatic cirrhosis were scanned by proteomic methods. We used two-dimensional electrophoresis (2-DE) and Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) to separate and identify the proteins which were differentially expressed in the serum of patients with hepatic fibrosis compared to HBV carriers. We identified 27 differentially expressed proteins, of which 19 proteins were up-regulated and 8 proteins were down-regulated in the serum of patients with hepatic fibrosis compared to HBV carriers. The expression level of enolase-1 (α-enolase) was decreased while the level of thrombospondin-1 (TSP-1) increased in the serum of patients with hepatic fibrosis by western blot. Enolase-1 and TSP-1 may be useful as biomarkers for the clinic diagnosis of hepatic fibrosis, but further study is necessary.

Keywords:
Hepatic fibrosis; Serum proteomics; HBV; Biomarker; Enolase-1; TSP-1