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Open Access Methodology

MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration

Juraj Lenco13, Renny Lan1, Nathan Edwards2 and Radoslav Goldman1*

Author Affiliations

1 Georgetown University, Department of Oncology, Lombardi Comprehensive Cancer Center, 3970 Reservoir Rd NW, Washington, DC 20057, USA

2 Georgetown University, Department of Biochemistry and Molecular and Cellular Biology, Washington, DC, USA

3 Current address: University Hospital Hradec Kralove; Center for Biomedical Research; Sokolska 581; 500 05 Hradec Kralove; Czech Republic

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Proteome Science 2012, 10:7  doi:10.1186/1477-5956-10-7

Published: 4 February 2012

Additional files

Additional file 1:

MSMS library.blib. Library of MS/MS spectra of serum native peptides that can be readily imported into the Skyline software for MRM transition design.

Format: XLSX Size: 20KB Download file

Open Data

Additional file 2:

Figure 1.pdf. Dilution curves for three native peptides and the respective CV values (n = 3).

Format: BLIB Size: 709KB Download file

Open Data