Methodology
MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration
1 Georgetown University, Department of Oncology, Lombardi Comprehensive Cancer Center, 3970 Reservoir Rd NW, Washington, DC 20057, USA
2 Georgetown University, Department of Biochemistry and Molecular and Cellular Biology, Washington, DC, USA
3 Current address: University Hospital Hradec Kralove; Center for Biomedical Research; Sokolska 581; 500 05 Hradec Kralove; Czech Republic
Proteome Science 2012, 10:7 doi:10.1186/1477-5956-10-7
Published: 4 February 2012Additional files
Additional file 1:
MSMS library.blib. Library of MS/MS spectra of serum native peptides that can be readily imported into the Skyline software for MRM transition design.
Format: XLSX Size: 20KB Download file
Additional file 2:
Figure 1.pdf. Dilution curves for three native peptides and the respective CV values (n = 3).
Format: BLIB Size: 709KB Download file
