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Magnetic bead-based salivary peptidome profiling for periodontal-orthodontic treatment

Jieni Zhang1, Shaonan Zhou1, Ruoxuan Li3, Tian Cao1, Hui Zheng1, Xuedong Wang1, Yanheng Zhou1, Ning Du2, Feng Chen2* and Jiuxiang Lin1*

Author Affiliations

1 Departments of Orthodontics and Central Laboratory, School of Stomatology, Peking University, #22 Zhongguancun South Road, Haidian District, Beijing, 100081, People's Republic of China

2 Department of Central Laboratory, School of Stomatology, Peking University, Beijing, 100081, People's Republic of China

3 Department of Stomatology, Beijing An Zhen Hospital, Capital University of Medical Sciences, Beijing, 100081, People's Republic of China

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Proteome Science 2012, 10:63  doi:10.1186/1477-5956-10-63

Published: 6 November 2012



Patients with periodontitis seek periodontal-orthodontic treatment to address certain functional and aesthetic problems. However, little is known of the effect of periodontitis on orthodontic treatment. Thus, we compared the differences in peptide mass fingerprints of orthodontic patients with and without periodontitis by MALDI-TOF MS using a magnetic bead-based peptidome analysis of saliva samples. In this way, we aimed to identify and explore a panel of differentially-expressed specific peptides.


Saliva samples from 24 patients (eight orthodontic patients without periodontitis, eight with periodontitis and another eight with periodontitis but no orthodontic treatment) were analyzed, and peptide mass fingerprints were created by scanning MS signals using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with magnetic beads. Nine mass peaks showed significant differences. Orthodontic patients in the group without periodontal disease showed higher mass peaks for seven peptides of the nine, whereas the mass peaks for the other two peptides were higher in the periodontal-orthodontic patients. Besides, these differentially-expressed peptides were sequenced.


The elucidated candidate biomarkers indicated interactions between periodontal condition and orthodontic treatment and their contributions to the changes of saliva protein profiles. Our results provide novel insight into the altered salivary protein profile during periodontal-orthodontic treatment, and may lead to the development of a therapeutic monitoring strategy for periodontics and orthodontics.

Periodontal-orthodontic treatment; Peptidome; Saliva; MALDI-TOF MS