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The defense response in Arabidopsis thaliana against Fusarium sporotrichioides

Tomoya Asano12, Makoto Kimura3 and Takumi Nishiuchi14*

Author Affiliations

1 Division of Functional Genomics, Advanced Science Research Center, Kanazawa University, 13-1 Takaramachi, Kanazawa, 920-0934, Japan

2 Equipment Support Promotion office, Advanced Science Research Center, Kanazawa University, Kanazawa, 920-1192, Japan

3 Plant & Microbial Metabolic Engineering Research Unit, Discovery Research Institute (DRI), RIKEN, 2–1 Hirosawa, Wako, Saitama, 351-0198, Japan

4 Division of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, 920-1192, Japan

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Proteome Science 2012, 10:61  doi:10.1186/1477-5956-10-61

Published: 30 October 2012

Abstract

Background

Certain graminaceous plants such as Zea mays and Triticum aestivum serve as hosts for Fusarium sporotrichioides; however, molecular interactions between the host plants and F. sporotrichioides remain unknown. It is also not known whether any interaction between Arabidopsis thaliana and F. sporotrichioides can occur. To understand these interactions, we performed proteomic analysis.

Results

Arabidopsis leaves and flowers were inoculated with F. sporotrichioides. Accumulation of PLANT DEFENSIN1.2 (PDF1.2) and PATHOGENESIS RELATED1 (PR1) mRNA in Arabidopsis were increased by inoculation of F. sporotrichioides. Furthermore, mitogen-activated protein kinase 3 (MPK3) and mitogen-activated protein kinase 6 (MPK6), which represent MAP kinases in Arabidopsis, were activated by inoculation of F. sporotrichioides. Proteomic analysis revealed that some defense-related proteins were upregulated, while the expression of photosynthesis- and metabolism-related proteins was down regulated, by inoculation with F. sporotrichioides. We carried out the proteomic analysis about upregulated proteins by inoculation with Fusarium graminearum. The glutathione S-transferases (GSTs), such as GSTF4 and GSTF7 were upregulated, by inoculation with F. graminearum-infected Arabidopsis leaves. On the other hand, GSTF3 and GSTF9 were uniquely upregulated, by inoculation with F. sporotrichioides.

Conclusions

These results indicate that Arabidopsis is a host plant for F. sporotrichioides. We revealed that defense response of Arabidopsis is initiated by infection with F. sporotrichioides.

Keywords:
Arabidopsis thaliana; Defense response; Fusarium sporotrichioides; T-2 toxin; MAP kinase; GSTs; Superoxide dismutase; Ascorbate peroxidase