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Changes in the proteomic profile of adipose tissue-derived mesenchymal stem cells during passages

Emanuele Capra14*, Riccardo Beretta1, Valentina Parazzi2, Mariele Viganò2, Lorenza Lazzari2, Antonella Baldi3 and Rosaria Giordano2

Author Affiliations

1 Biotrack S.r.l, Parco Tecnologico Padano, Lodi, Italy

2 Department of Regenerative Medicine, Cell Factory, Center of Transfusion Medicine, Cellular Therapy and Cryobiology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, Italy

3 Department of Veterinary Science and Technologies for Food Safety, Università degli Studi di Milano, Milan, Italy

4 Institute Biotrack S.r.l, Via Einstein 26900, Lodi, Italy

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Proteome Science 2012, 10:46  doi:10.1186/1477-5956-10-46

Published: 24 July 2012

Abstract

Background

Human mesenchymal stem cells (hMSC) have recently raised the attention because of their therapeutic potential in the novel context of regenerative medicine. However, the safety of these new and promising cellular products should be carefully defined before they can be used in the clinical setting, as. The protein expression profile of these cells might reveal potential hazards associated with senescence and tumoral transformation which may occur during culture. Proteomic is a valuable tool for hMSC characterization and identification of possible changes during expansion.

Results

We used Surface Enhanced Laser Desorption/Ionization-Time Of Flight-Mass Spectrometry (SELDI-ToF-MS) to evaluate the presence of stable molecular markers in adipose tissue-derived mesenchymal stem cells (AD-MSC) produced under conditions of good manufacturing practices (GMP). Proteomic patterns of cells prepared were consistent, with 4 up-regulated peaks (mass-to-charge ratio (m/z) 8950, 10087, 10345, and 13058) through subculture steps (P0-P7) with similar trend in three donors. Among the differentially expressed proteins found in the cytoplasmic and nuclear fractions, a cytoplasmic 10.1 kDa protein was upregulated during culture passages and was identified as S100A6 (Calcyclin).

Conclusions

This study suggests for the first time that common variation could occur in AD-MSC from different donors, with the identification of S100A6, a protein prevalently related to cell proliferation and cell culture condition. These results support the hypothesis of common proteomic changes during MSCs expansion and could give important insight in the knowledge of molecular mechanisms intervening during MSC expansion.

Keywords:
Adipose tissue-derived mesenchymal stem cells; AD-MSC; S-100A6; Calcyclin; SELDI-ToF; Proteomic