Email updates

Keep up to date with the latest news and content from Proteome Science and BioMed Central.

Open Access Highly Accessed Research

A proteome map of primary cultured rat Schwann cells

Mi Shen1, Yuhua Ji12, Shuqiang Zhang1, Haiyan Shi1, Gang Chen1, Xiaosong Gu1 and Fei Ding1*

Author Affiliations

1 Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu Province 226001, Peoples' Republic of China

2 Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou, China

For all author emails, please log on.

Proteome Science 2012, 10:20  doi:10.1186/1477-5956-10-20

Published: 23 March 2012

Abstract

Background

Schwann cells (SCs) are the principal glial cells of the peripheral nervous system with a wide range of biological functions. SCs play a key role in peripheral nerve regeneration and are involved in several hereditary peripheral neuropathies. The objective of this study was to gain new insight into the whole protein composition of SCs.

Results

Two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D LC-MS/MS) was performed to identify the protein expressions in primary cultured SCs of rats. We identified a total of 1,232 proteins, which were categorized into 20 functional classes. We also used quantitative real time RT-PCR and Western blot analysis to validate some of proteomics-identified proteins.

Conclusion

We showed for the first time the proteome map of SCs. Our data could serve as a reference library to provide basic information for understanding SC biology.

Keywords:
Schwann cell; Proteome; 2D LC/MS/MS