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Open Access Research

Treatment with captopril abrogates the altered expression of alpha1 macroglobulin and alpha1 antiproteinase in sera of spontaneously hypertensive rats

Norhaniza Aminudin12*, Nur-Atiqah H Abdullah1, Hasni Misbah3, Saiful A Karsani12, Ruby Husain4, See Z Hoe4 and Onn H Hashim23

Author Affiliations

1 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

2 University of Malaya Centre for Proteomics Research, University of Malaya, Kuala Lumpur, Malaysia

3 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

4 Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

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Proteome Science 2012, 10:17  doi:10.1186/1477-5956-10-17

Published: 15 March 2012

Abstract

Background

Proteins that are associated with hypertension may be identified by comparing the 2-dimensional gel electrophoresis (2-DE) profiles of the sera of spontaneously hypertensive rats (SHR) with those generated from normotensive Spraque-Dawley rats (SDR).

Results

Five proteins of high abundance were found to be significantly altered when the 2-DE serum profiles of the SHR were compared to those that were similarly generated from the SDR. Analysis by mass spectrometry and database search identified the proteins as retinol binding protein 4, complement C3, albumin (19.9 kDa fragment), alpha1 macroglobulin and alpha1 antiproteinase, which are all known to be associated with hypertension. The altered expression of the two latter proteins was found to be abrogated when similar analysis was performed on sera of the SHR that were treated with captopril.

Conclusion

Our data suggests that serum alpha1 macroglobulin and alpha1 antiproteinase are potentially useful complementary biomolecular indicators for monitoring of hypertension.

Keywords:
hypertension; biomarker; spontaneously hypertensive rats; serum proteins; proteomics